Restriction map for
pFN21AE2557
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccIII AclI AflIII AgeI Aor51HI
AscI AseI AsnI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BlnI BsaAI BsaBI BseAI BsiWI
BsmBI BspEI BspHI BspLU11I BssHII
BssSI BstBI CpoI DraI DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 1270 GACGTC GACGT^C
Acc65I 1593 GGTACC G^GTACC
AccI 2661 GTATAC GT^[AC][GT]AC
AccII 667 CGCG CG^CG
AflII 2527 CTTAAG C^TTAAG
AhdI 907 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1081 GTGCAC G^TGCAC
ApaLI 1081 GTGCAC G^TGCAC
Asp700I 2625 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1593 GGTACC G^GTACC
AspEI 907 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1099 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1773 TGGCCA TGG^CCA
BbsI 376 GAAGAC GAAGAC(2/6)
BciVI 1927 GGATAC GTATCC(6/5)
BclI 1740 TGATCA T^GATCA
BfrI 2527 CTTAAG C^TTAAG
BglI 1979 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 158 AGATCT A^GATCT
BpuAI 376 GAAGAC GAAGAC(2/6)
BsmI 412 GAATGC GAATGC(1/-1)
Bsp1407I 1967 TGTACA T^GTACA
BspDI 1762 ATCGAT AT^CGAT
BsrDI 531 CATTGC GCAATG(2/0)
BsrGI 1967 TGTACA T^GTACA
Bst1107I 2661 GTATAC GTA^TAC
BstAPI 1851 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 630 GGTGACC G^GT[ACGT]ACC
BstPI 630 GGTGACC G^GT[ACGT]ACC
BstUI 667 CGCG CG^CG
BstZ17I 2661 GTATAC GTA^TAC
ClaI 1762 ATCGAT AT^CGAT
DraIII 1084 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1773 TGGCCA [CT]^GGCC[GA]
Eam1105I 907 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 630 GGTGACC G^GT[ACGT]ACC
EcoRI 2431 GAATTC G^AATTC
FbaI 1740 TGATCA T^GATCA
KpnI 1593 GGTACC GGTAC^C
MluNI 1773 TGGCCA TGG^CCA
MscI 1773 TGGCCA TGG^CCA
MvnI 667 CGCG CG^CG
NheI 150 GCTAGC G^CTAGC
PflFI 1099 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2836 GAGCTC GAGCT^C
SfaNI 2360 GCATC GCATC(5/9)
SmaI 2561 CCCGGG CCC^GGG
SspBI 1967 TGTACA T^GTACA
StuI 2087 AGGCCT AGG^CCT
Tth111I 1099 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2561 CCCGGG C^CCGGG
XmaI 2561 CCCGGG C^CCGGG
XmnI 2625 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 2431 GAATTC [GA]^AATT[CT]
2709 GAATTT
AcyI 1270 GACGTC G[GA]^CG[CT]C
2314 GGCGTC
AlwNI 1186 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1505 CAGGGGCTG
ApoI 2431 GAATTC [GA]^AATT[CT]
2709 GAATTT
BfaI 151 CTAG C^TAG
1547 CTAG
BlpI 1110 GCTGAGC GC^T[ACGT]AGC
2733 GCTCAGC
Bpu1102I 1110 GCTGAGC GC^T[ACGT]AGC
2733 GCTCAGC
BsaHI 1270 GACGTC G[GA]^CG[CT]C
2314 GGCGTC
BseRI 2271 CTCCTC GAGGAG(10/8)
102 GAGGAG
BsiEI 69 CGACCG CG[GA][CT]^CG
1311 CGACCG
BsrBI 1942 GAGCGG CCGCTC(-3/-3)
2782 GAGCGG
BstYI 158 AGATCT [GA]^GATC[CT]
2502 GGATCT
CelII 1110 GCTGAGC GC^T[ACGT]AGC
2733 GCTCAGC
EarI 334 CTCTTC CTCTTC(1/4)
2224 GAAGAG
EcoNI 2694 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2772 CCTCCCCCAGG
EcoRV 124 GATATC GAT^ATC
892 GATATC
EcoT14I 2436 CCAAGG C^C[AT][AT]GG
2567 CCAAGG
HaeII 699 GGCGCT [GA]GCGC^[CT]
1226 GGCGCT
Hin1I 1270 GACGTC G[GA]^CG[CT]C
2314 GGCGTC
Ksp632I 334 CTCTTC CTCTTC(1/4)
2224 GAAGAG
MaeI 151 CTAG C^TAG
1547 CTAG
MflI 158 AGATCT [GA]^GATC[CT]
2502 GGATCT
NaeI 517 GCCGGC GCC^GGC
1708 GCCGGC
NgoMIV 517 GCCGGC G^CCGGC
1708 GCCGGC
PshAI 45 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1312 GACCGGAGTC
SmlI 1300 CTCAAG C^T[CT][GA]AG
2527 CTTAAG
StyI 2436 CCAAGG C^C[AT][AT]GG
2567 CCAAGG
XhoII 158 AGATCT [GA]^GATC[CT]
2502 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I