Restriction map for
pFN21AA0213
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# NO CUT ENZYMES ( 71 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI BbeI BbrPI BciVI BfrI
BglI BglII BsaHI BseAI BsiWI
BsmBI BspEI BsrBI BssHII Bst1107I
BstAPI BstZ17I CpoI DraIII DrdI
Eco47III EcoRV FseI HaeII Hin1I
HpaI KasI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RsrII SacII SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Van91I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AocI 2785 CCTCAGG CC^T[ACGT]AGG
AviII 2856 TGCGCA TGC^GCA
AvrII 1405 CCTAGG C^CTAGG
BamHI 2123 GGATCC G^GATCC
BbsI 205 GAAGAC GAAGAC(2/6)
BclI 465 TGATCA T^GATCA
BlnI 1405 CCTAGG C^CTAGG
BlpI 586 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 586 GCTAAGC GC^T[ACGT]AGC
BpuAI 205 GAAGAC GAAGAC(2/6)
BsaAI 1670 CACGTA [CT]AC^GT[GA]
BspHI 1020 TCATGA T^CATGA
BsrFI 2940 GCCGGT [GA]^CCGG[CT]
BssSI 4394 CTCGTG CACGAG(-5/-1)
BstEII 3864 GGTGACC G^GT[ACGT]ACC
BstPI 3864 GGTGACC G^GT[ACGT]ACC
BstXI 4212 CCATGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2785 CCTCAGG CC^T[ACGT]AGG
CelII 586 GCTAAGC GC^T[ACGT]AGC
Cfr10I 2940 GCCGGT [GA]^CCGG[CT]
EagI 4424 CGGCCG C^GGCCG
EclXI 4424 CGGCCG C^GGCCG
Eco52I 4424 CGGCCG C^GGCCG
Eco81I 2785 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3864 GGTGACC G^GT[ACGT]ACC
EcoRI 2491 GAATTC G^AATTC
FbaI 465 TGATCA T^GATCA
FspI 2856 TGCGCA TGC^GCA
MfeI 3302 CAATTG C^AATTG
MunI 3302 CAATTG C^AATTG
PvuI 1692 CGATCG CGAT^CG
RcaI 1020 TCATGA T^CATGA
SspI 4026 AATATT AAT^ATT
StuI 4477 AGGCCT AGG^CCT
XbaI 201 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 35 enzymes) #
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AflIII 3029 ACATGT A^C[GA][CT]GT
4161 ACATGT
AlwNI 596 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
2962 CAGCAGCTG
AseI 883 ATTAAT AT^TAAT
4275 ATTAAT
AsnI 883 ATTAAT AT^TAAT
4275 ATTAAT
AspI 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1556 GACATTGTC
AvaI 112 CCCGAG C^[CT]CG[GA]G
130 CCCGAG
BcgI 2715 GCACGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3929 GCATCAGCGTCG
BsaBI 541 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2692 GATGCCTATC
BsaWI 105 ACCGGA [AT]^CCGG[AT]
1413 ACCGGA
BsiEI 1692 CGATCG CG[GA][CT]^CG
4424 CGGCCG
BsoBI 112 CCCGAG C^[CT]CG[GA]G
130 CCCGAG
BspDI 2689 ATCGAT AT^CGAT
3548 ATCGAT
BspLU11I 3029 ACATGT A^CATGT
4161 ACATGT
BstBI 1834 TTCGAA TT^CGAA
4030 TTCGAA
CfoI 19 GCGC GCG^C
2857 GCGC
ClaI 2689 ATCGAT AT^CGAT
3548 ATCGAT
DraI 666 TTTAAA TTT^AAA
2163 TTTAAA
EcoNI 1658 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3406 CCTGTTACAGG
EcoT22I 2978 ATGCAT ATGCA^T
3372 ATGCAT
FauI 34 CCCGC CCCGC(4/6)
1458 GCGGG
HgaI 2062 GACGC GACGC(5/10)
3935 GCGTC
HhaI 19 GCGC GCG^C
2857 GCGC
HinP1I 19 GCGC G^CGC
2857 GCGC
MamI 541 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2692 GATGCCTATC
NsiI 2978 ATGCAT ATGCA^T
3372 ATGCAT
NspV 1834 TTCGAA TT^CGAA
4030 TTCGAA
PflFI 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1556 GACATTGTC
PshBI 883 ATTAAT AT^TAAT
4275 ATTAAT
SacI 2407 GAGCTC GAGCT^C
4075 GAGCTC
SalI 907 GTCGAC G^TCGAC
3937 GTCGAC
SapI 1117 GCTCTTC GCTCTTC(1/4)
2409 GCTCTTC
SfuI 1834 TTCGAA TT^CGAA
4030 TTCGAA
SpeI 2335 ACTAGT A^CTAGT
2838 ACTAGT
SphI 813 GCATGC GCATG^C
2816 GCATGC
Tth111I 117 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1556 GACATTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
Acc65I AccI AccII AciI AcsI
AfaI AhdI AluI AlwI ApoI
Asp700I Asp718I AspEI AspHI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI Eam1105I EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI