Restriction map for
pFN21AB6564
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspHI AspI AviII AvrII
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsiHKAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BsrI BssHII Bst1107I BstZ17I
Bsu36I CelII ClaI DraI DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI Ksp632I KspI MaeII
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
SacI SacII SapI SbfI ScaI
SfiI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I Van91I XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 791 GGTACC G^GTACC
AccI 850 GTCGAC GT^[AC][GT]AC
ApaI 60 GGGCCC GGGCC^C
Asp700I 163 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 791 GGTACC G^GTACC
BanII 60 GGGCCC G[GA]GC[CT]^C
BfaI 611 CTAG C^TAG
BsiEI 889 CGGCCG CG[GA][CT]^CG
BsmFI 765 GTCCC GGGAC(10/14)
BsmI 370 GAATGC GAATGC(1/-1)
BssSI 601 CTCGTG CACGAG(-5/-1)
BstAPI 357 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 214 TTCGAA TT^CGAA
BstEII 255 GGTCACC G^GT[ACGT]ACC
BstPI 255 GGTCACC G^GT[ACGT]ACC
BstXI 815 CCAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 1111 CGGTCCG CG^G[AT]CCG
EagI 889 CGGCCG C^GGCCG
EclXI 889 CGGCCG C^GGCCG
Eco52I 889 CGGCCG C^GGCCG
EcoNI 462 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 255 GGTCACC G^GT[ACGT]ACC
FseI 838 GGCCGGCC GGCCGG^CC
HgaI 811 GACGC GACGC(5/10)
HincII 850 GTCGAC GT[CT]^[GA]AC
HindII 850 GTCGAC GT[CT]^[GA]AC
KpnI 791 GGTACC GGTAC^C
MaeI 611 CTAG C^TAG
NspV 214 TTCGAA TT^CGAA
PleI 661 GACTC GAGTC(4/5)
PpuMI 359 AGGTCCT [GA]G^G[AT]CC[CT]
RsrII 1111 CGGTCCG CG^G[AT]CCG
SalI 850 GTCGAC G^TCGAC
SfuI 214 TTCGAA TT^CGAA
SmaI 901 CCCGGG CCC^GGG
SmlI 313 CTTGAG C^T[CT][GA]AG
TfiI 492 GATTC G^A[AT]TC
TspRI 414 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 610 TCTAGA T^CTAGA
XcmI 671 CCAACTCCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 901 CCCGGG C^CCGGG
XmaI 901 CCCGGG C^CCGGG
XmnI 163 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 332 TCCGGA T^CCGGA
705 TCCGGA
AlwNI 358 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
469 CAGGTGCTG
BbeI 82 GGCGCC GGCGC^C
905 GGCGCC
BglI 85 GCCTCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
897 GCCCCCCGGGC
BsaWI 332 TCCGGA [AT]^CCGG[AT]
705 TCCGGA
BseAI 332 TCCGGA T^CCGGA
705 TCCGGA
BseRI 124 GAGGAG GAGGAG(10/8)
499 GAGGAG
BsgI 364 CTGCAC GTGCAG(16/14)
736 GTGCAG
BspEI 332 TCCGGA T^CCGGA
705 TCCGGA
BspMI 107 ACCTGC ACCTGC(4/8)
357 GCAGGT
BstYI 221 GGATCT [GA]^GATC[CT]
503 AGATCC
DdeI 225 CTCAG C^T[ACGT]AG
992 CTAAG
HinfI 492 GATTC G^A[ACGT]TC
661 GACTC
KasI 82 GGCGCC G^GCGCC
905 GGCGCC
MflI 221 GGATCT [GA]^GATC[CT]
503 AGATCC
MroI 332 TCCGGA T^CCGGA
705 TCCGGA
MspA1I 915 CAGCGG C[AC]G^C[GT]G
1061 CAGCGG
NarI 82 GGCGCC GG^CGCC
905 GGCGCC
NcoI 665 CCATGG C^CATGG
944 CCATGG
PstI 109 CTGCAG CTGCA^G
355 CTGCAG
SexAI 98 ACCTGGT A^CC[AT]GGT
467 ACCAGGT
SfoI 82 GGCGCC GGC^GCC
905 GGCGCC
TaqI 215 TCGA T^CGA
851 TCGA
TthHB8I 215 TCGA T^CGA
851 TCGA
XhoII 221 GGATCT [GA]^GATC[CT]
503 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BalI BanI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsiYI BslI BsoBI Bsp1286I
BsrBI BsrFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MaeIII
MboI MboII MluNI MnlI MscI
MslI MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI Tsp45I