Restriction map for
pFN21AE3681
##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbsI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III Eco81I EcoRI EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI Ksp632I MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 669 GACGTC GACGT^C
Alw44I 988 GTGCAC G^TGCAC
AlwI 228 GATCC GGATC(4/5)
ApaLI 988 GTGCAC G^TGCAC
BbeI 861 GGCGCC GGCGC^C
BbrPI 271 CACGTG CAC^GTG
BcgI 619 GCAGTGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 210 CTCCAG CTGGAG(16/14)
BsaAI 271 CACGTG [CT]AC^GT[GA]
BsgI 949 CTGCAC GTGCAG(16/14)
BsiEI 402 CGGCCG CG[GA][CT]^CG
BssSI 480 CTCGTG CACGAG(-5/-1)
BstAPI 693 GCAGGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 942 GGTCACC G^GT[ACGT]ACC
BstPI 942 GGTCACC G^GT[ACGT]ACC
DraII 86 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 841 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 402 CGGCCG C^GGCCG
EclXI 402 CGGCCG C^GGCCG
Eco52I 402 CGGCCG C^GGCCG
EcoNI 519 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 86 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 942 GGTCACC G^GT[ACGT]ACC
EcoRV 239 GATATC GAT^ATC
EcoT14I 65 CCATGG C^C[AT][AT]GG
KasI 861 GGCGCC G^GCGCC
KspI 680 CCGCGG CCGC^GG
MaeIII 943 GTCAC ^GT[ACGT]AC
MboII 350 TCTTC GAAGA(8/7)
MslI 786 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 861 GGCGCC GG^CGCC
NcoI 65 CCATGG C^CATGG
PflMI 419 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 271 CACGTG CAC^GTG
PmlI 271 CACGTG CAC^GTG
PshAI 112 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 680 CCGCGG CCGC^GG
SfaNI 545 GCATC GCATC(5/9)
SfoI 861 GGCGCC GGC^GCC
StuI 122 AGGCCT AGG^CCT
StyI 65 CCATGG C^C[AT][AT]GG
TfiI 377 GATTC G^A[AT]TC
Tsp45I 943 GTCAC ^GT[GC]AC
Van91I 419 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 492 CCAGCTGTCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 278 GGTGCC G^G[CT][GA]CC
861 GGCGCC
BanII 98 GAGCTC G[GA]GC[CT]^C
437 GAGCTC
BbvCI 771 CCTCAGC CCTCAGC(-5/-2)
309 GCTGAGG
BglII 347 AGATCT A^GATCT
410 AGATCT
BspMI 693 GCAGGT ACCTGC(4/8)
984 GCAGGT
BsrBI 1048 CCGCTC CCGCTC(-3/-3)
928 GAGCGG
BsrFI 651 GCCGGC [GA]^CCGG[CT]
995 GCCGGC
Cfr10I 651 GCCGGC [GA]^CCGG[CT]
995 GCCGGC
DsaI 65 CCATGG C^C[GA][CT]GG
680 CCGCGG
EaeI 402 CGGCCG [CT]^GGCC[GA]
653 CGGCCA
HaeII 488 AGCGCC [GA]GCGC^[CT]
861 GGCGCC
HincII 253 GTCAAC GT[CT]^[GA]AC
641 GTCAAC
HindII 253 GTCAAC GT[CT]^[GA]AC
641 GTCAAC
NaeI 651 GCCGGC GCC^GGC
995 GCCGGC
NgoMIV 651 GCCGGC G^CCGGC
995 GCCGGC
PaeR7I 223 CTCGAG C^TCGAG
283 CTCGAG
PleI 782 GAGTC GAGTC(4/5)
966 GAGTC
SacI 98 GAGCTC GAGCT^C
437 GAGCTC
XhoI 223 CTCGAG C^TCGAG
283 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaI AvaII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MboI MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI SmlI TaqI TseI
TspRI TthHB8I XhoII