##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AciI          362  GCGG                 CCGC(-3/-1)
AcsI          274  AAATTC               [GA]^AATT[CT]
AflIII        371  ACATGT               A^C[GA][CT]GT
AlwNI         186  CAGCAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          274  AAATTC               [GA]^AATT[CT]
AseI           97  ATTAAT               AT^TAAT
AsnI           97  ATTAAT               AT^TAAT
BcnI           40  CCGGG                CC^[GC]GG
BglI          291  GCCAGATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          298  GGGCAC               G[AGT]GC[ACT]^C
BsmFI         112  GGGAC                GGGAC(10/14)
Bsp1286I      298  GGGCAC               G[AGT]GC[ACT]^C
BspLU11I      371  ACATGT               A^CATGT
BspMI         128  ACCTGC               ACCTGC(4/8)
BsrI          191  ACTGG                ACTGG(1/-1)
BssSI         301  CACGAG               CACGAG(-5/-1)
BstXI         317  CCAACAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I        105  GGGCC                G^G[ACGT]CC
FauI          362  GCGGG                CCCGC(4/6)
HaeIII        106  GGCC                 GG^CC
HapII          40  CCGG                 C^CGG
HincII         45  GTCAAC               GT[CT]^[GA]AC
HindII         45  GTCAAC               GT[CT]^[GA]AC
HinfI         154  GAATC                G^A[ACGT]TC
HpaII          40  CCGG                 C^CGG
MslI           27  CATGGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           40  CCGG                 C^CGG
NciI           40  CCGGG                CC^[GC]GG
NspI          371  ACATGT               [GA]CATG^[CT]
PshBI          97  ATTAAT               AT^TAAT
Sau96I        105  GGGCC                G^G[ACGT]CC
SfaNI          68  GCATC                GCATC(5/9)
SmlI           13  CTTGAG               C^T[CT][GA]AG
TfiI          154  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BstNI         108  CCAGG                CC^[AT]GG
              232  CCAGG               
DdeI          340  CTGAG                C^T[ACGT]AG
              349  CTGAG               
DpnII          57  GATC                 ^GATC
              214  GATC                
EcoRII        108  CCAGG                ^CC[AT]GG
              232  CCAGG               
HphI          282  GGTGA                GGTGA(8/7)
              216  TCACC               
MboI           57  GATC                 ^GATC
              214  GATC                
MboII          54  GAAGA                GAAGA(8/7)
              211  GAAGA               
MvaI          108  CCAGG                CC^[AT]GG
              232  CCAGG               
NdeII          57  GATC                 ^GATC
              214  GATC                
Sau3AI         57  GATC                 ^GATC
              214  GATC                
Tsp509I        96  AATT                 ^AATT
              275  AATT                
TspRI          81  CACTG                CA[GC]TG[ACGT][ACGT]^
              254  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BbvI        BsaJI       BssKI       Fnu4HI      
ItaI        MaeIII      MnlI        MseI        MwoI        
NlaIII      ScrFI       Tru9I       TseI        Tsp45I