Restriction map for
pFN21AE6102
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AluI 230 AGCT AG^CT
AocI 244 CCTGAGG CC^T[ACGT]AGG
AspHI 229 GAGCTC G[AT]GC[AT]^C
BanII 229 GAGCTC G[GA]GC[CT]^C
BbvCI 96 CCTCAGC CCTCAGC(-5/-2)
BbvI 161 GCTGC GCAGC(8/12)
BglII 128 AGATCT A^GATCT
BmyI 229 GAGCTC G[AGT]GC[ACT]^C
BsaJI 80 CCATGG C^C[ACGT][ACGT]GG
BsaWI 233 TCCGGT [AT]^CCGG[AT]
BsiHKAI 229 GAGCTC G[AT]GC[AT]^C
BsmAI 289 GAGAC GTCTC(1/5)
Bsp1286I 229 GAGCTC G[AGT]GC[ACT]^C
BsrBI 222 GAGCGG CCGCTC(-3/-3)
BstXI 188 CCATAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 128 AGATCT [GA]^GATC[CT]
Bsu36I 244 CCTGAGG CC^T[ACGT]AGG
DsaI 80 CCATGG C^C[GA][CT]GG
EaeI 44 CGGCCA [CT]^GGCC[GA]
EarI 286 GAAGAG CTCTTC(1/4)
Eco81I 244 CCTGAGG CC^T[ACGT]AGG
EcoNI 255 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 80 CCATGG C^C[AT][AT]GG
HapII 234 CCGG C^CGG
HpaII 234 CCGG C^CGG
HphI 60 GGTGA GGTGA(8/7)
Ksp632I 286 GAAGAG CTCTTC(1/4)
MflI 128 AGATCT [GA]^GATC[CT]
MslI 189 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 159 CCGCTG C[AC]G^C[GT]G
MspI 234 CCGG C^CGG
NcoI 80 CCATGG C^CATGG
NlaIII 81 CATG CATG^
PflMI 75 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 229 GAGCTC GAGCT^C
StyI 80 CCATGG C^C[AT][AT]GG
TseI 161 GCTGC G^C[AT]GC
TspRI 22 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 75 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 128 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AvaII 174 GGACC G^G[AT]CC
258 GGTCC
BseRI 85 GAGGAG GAGGAG(10/8)
247 GAGGAG
BssKI 255 CCTGG ^CC[ACGT]GG
261 CCAGG
BstF5I 111 GGATG GGATG(2/0)
267 GGATG
BstNI 255 CCTGG CC^[AT]GG
261 CCAGG
EcoRII 255 CCTGG ^CC[AT]GG
261 CCAGG
FauI 104 CCCGC CCCGC(4/6)
224 GCGGG
Fnu4HI 158 GCCGC GC^[ACGT]GC
161 GCTGC
FokI 111 GGATG GGATG(9/13)
267 GGATG
ItaI 158 GCCGC GC^[ACGT]GC
161 GCTGC
MboII 286 GAAGA GAAGA(8/7)
131 TCTTC
MvaI 255 CCTGG CC^[AT]GG
261 CCAGG
MwoI 107 GCAAGGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
231 GCTCCGGTGGC
NlaIV 198 GGAACC GG[ACGT]^[ACGT]CC
239 GGCCCC
ScrFI 255 CCTGG CC^[ACGT]GG
261 CCAGG
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
AciI BsiYI BslI Cfr13I DdeI
DpnII HaeIII MboI MnlI NdeII
Sau3AI Sau96I