Restriction map for
pFN21AA1354
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BglI
BlnI BlpI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HapII HincII
HindII HpaI HpaII KasI Ksp632I
KspI MamI MluI MroI MspI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 162 GGTACC G^GTACC
AciI 1586 CCGC CCGC(-3/-1)
AcsI 801 GAATTT [GA]^AATT[CT]
AflII 619 CTTAAG C^TTAAG
AlwNI 925 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 801 GAATTT [GA]^AATT[CT]
Asp718I 162 GGTACC G^GTACC
AspHI 1384 GAGCTC G[AT]GC[AT]^C
AvaII 1574 GGACC G^G[AT]CC
BalI 1238 TGGCCA TGG^CCA
BanI 162 GGTACC G^G[CT][GA]CC
BanII 1384 GAGCTC G[GA]GC[CT]^C
BbsI 676 GAAGAC GAAGAC(2/6)
BclI 120 TGATCA T^GATCA
BfrI 619 CTTAAG C^TTAAG
BglII 982 AGATCT A^GATCT
BpuAI 676 GAAGAC GAAGAC(2/6)
BsaAI 757 TACGTG [CT]AC^GT[GA]
BseRI 911 GAGGAG GAGGAG(10/8)
BsiHKAI 1384 GAGCTC G[AT]GC[AT]^C
BsmBI 33 CGTCTC CGTCTC(1/5)
BsmFI 9 GTCCC GGGAC(10/14)
BsmI 1757 GCATTC GAATGC(1/-1)
BspHI 776 TCATGA T^CATGA
BsrDI 1456 CATTGC GCAATG(2/0)
BstEII 1805 GGTCACC G^GT[ACGT]ACC
BstPI 1805 GGTCACC G^GT[ACGT]ACC
BstXI 890 CCACTCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 1574 GGACC G^G[ACGT]CC
DraIII 1748 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1238 TGGCCA [CT]^GGCC[GA]
EcoO65I 1805 GGTCACC G^GT[ACGT]ACC
EcoT14I 12 CCTTGG C^C[AT][AT]GG
FbaI 120 TGATCA T^GATCA
HindIII 280 AAGCTT A^AGCTT
KpnI 162 GGTACC GGTAC^C
MaeII 758 ACGT A^CGT
MfeI 1580 CAATTG C^AATTG
MluNI 1238 TGGCCA TGG^CCA
MscI 1238 TGGCCA TGG^CCA
MunI 1580 CAATTG C^AATTG
NspI 1460 GCATGT [GA]CATG^[CT]
PflMI 166 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1437 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 776 TCATGA T^CATGA
SacI 1384 GAGCTC GAGCT^C
Sau96I 1574 GGACC G^G[ACGT]CC
ScaI 556 AGTACT AGT^ACT
SexAI 165 ACCAGGT A^CC[AT]GGT
SfcI 1439 CTACAG C^T[GA][CT]AG
StyI 12 CCTTGG C^C[AT][AT]GG
TfiI 1819 GAATC G^A[AT]TC
Van91I 166 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1813 TCTAGA T^CTAGA
XcmI 1602 CCAAAGTGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 31 GGCGTC G[GA]^CG[CT]C
210 GGCGTC
AlwI 1117 GATCC GGATC(4/5)
744 GGATC
BsaHI 31 GGCGTC G[GA]^CG[CT]C
210 GGCGTC
BsgI 760 GTGCAG GTGCAG(16/14)
1223 GTGCAG
BsmAI 34 GTCTC GTCTC(1/5)
445 GTCTC
Bsp1407I 1465 TGTACA T^GTACA
1768 TGTACA
BspMI 793 ACCTGC ACCTGC(4/8)
1140 GCAGGT
BsrGI 1465 TGTACA T^GTACA
1768 TGTACA
BstYI 744 GGATCT [GA]^GATC[CT]
982 AGATCT
CfoI 1164 GCGC GCG^C
1218 GCGC
HaeIII 1239 GGCC GG^CC
1600 GGCC
HgaI 32 GCGTC GACGC(5/10)
211 GCGTC
HhaI 1164 GCGC GCG^C
1218 GCGC
Hin1I 31 GGCGTC G[GA]^CG[CT]C
210 GGCGTC
HinP1I 1164 GCGC G^CGC
1218 GCGC
MflI 744 GGATCT [GA]^GATC[CT]
982 AGATCT
MspA1I 928 CAGCTG C[AC]G^C[GT]G
1226 CAGCTG
NheI 385 GCTAGC G^CTAGC
817 GCTAGC
PvuII 928 CAGCTG CAG^CTG
1226 CAGCTG
SmlI 619 CTTAAG C^T[CT][GA]AG
1179 CTTGAG
SspBI 1465 TGTACA T^GTACA
1768 TGTACA
SspI 399 AATATT AAT^ATT
715 AATATT
XhoII 744 GGATCT [GA]^GATC[CT]
982 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI BbvI BfaI BmyI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HinfI
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI ScrFI SfaNI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I