Restriction map for
pFN21AB8412
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstZ17I
Bsu36I ClaI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MslI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
XhoI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AviII 1161 TGCGCA TGC^GCA
BalI 113 TGGCCA TGG^CCA
BbsI 169 GTCTTC GAAGAC(2/6)
BcgI 923 CGAAGGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 169 GTCTTC GAAGAC(2/6)
BsiEI 2472 CGGCCG CG[GA][CT]^CG
BsmBI 1009 GAGACG CGTCTC(1/5)
BsrBI 1188 GAGCGG CCGCTC(-3/-3)
BssHII 2451 GCGCGC G^CGCGC
BstBI 921 TTCGAA TT^CGAA
BstEII 2244 GGTGACC G^GT[ACGT]ACC
BstPI 2244 GGTGACC G^GT[ACGT]ACC
CpoI 2020 CGGTCCG CG^G[AT]CCG
DsaI 1560 CCATGG C^C[GA][CT]GG
EagI 2472 CGGCCG C^GGCCG
EclXI 2472 CGGCCG C^GGCCG
Eco52I 2472 CGGCCG C^GGCCG
EcoNI 2310 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2244 GGTGACC G^GT[ACGT]ACC
FspI 1161 TGCGCA TGC^GCA
MaeII 2432 ACGT A^CGT
MluNI 113 TGGCCA TGG^CCA
MscI 113 TGGCCA TGG^CCA
NcoI 1560 CCATGG C^CATGG
NspV 921 TTCGAA TT^CGAA
RsrII 2020 CGGTCCG CG^G[AT]CCG
SfuI 921 TTCGAA TT^CGAA
SgrAI 2161 CACCGGCG C[GA]^CCGG[CT]G
SphI 974 GCATGC GCATG^C
Tsp509I 959 AATT ^AATT
XbaI 1432 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 2452 CGCG CG^CG
2454 CGCG
AcyI 1011 GACGCC G[GA]^CG[CT]C
2681 GGCGTC
BglI 2288 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2623 GCCCGGACGGC
BsaHI 1011 GACGCC G[GA]^CG[CT]C
2681 GGCGTC
BsgI 517 CTGCAC GTGCAG(16/14)
2210 GTGCAG
BsmI 132 GAATGC GAATGC(1/-1)
324 GAATGC
BstUI 2452 CGCG CG^CG
2454 CGCG
BstXI 2075 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2222 CCAAGCAGCTGG
Hin1I 1011 GACGCC G[GA]^CG[CT]C
2681 GGCGTC
MvnI 2452 CGCG CG^CG
2454 CGCG
NaeI 2474 GCCGGC GCC^GGC
2754 GCCGGC
NgoMIV 2474 GCCGGC G^CCGGC
2754 GCCGGC
PpuMI 1239 GGGACCC [GA]G^G[AT]CC[CT]
1922 AGGACCT
SacI 428 GAGCTC GAGCT^C
2671 GAGCTC
SapI 2643 GAAGAGC GCTCTTC(1/4)
2325 GCTCTTC
TaqI 922 TCGA T^CGA
1112 TCGA
TfiI 462 GATTC G^A[AT]TC
2008 GATTC
TthHB8I 922 TCGA T^CGA
1112 TCGA
XcmI 1644 CCAGAGGGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2558 CCAATAAGACGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BamHI
BanI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI Tru9I TseI
Tsp45I TspRI Van91I XhoII XmaCI
XmaI