Restriction map for
pFN21AE9463
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsiYI BslI
BsoBI Bsp1407I BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI Sau96I SbfI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 753 GCGG CCGC(-3/-1)
AfaI 69 GTAC GT^AC
AluI 210 AGCT AG^CT
AlwNI 50 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 388 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 431 TGGCCA TGG^CCA
BanI 505 GGCACC G^G[CT][GA]CC
BanII 968 GGGCTC G[GA]GC[CT]^C
BbsI 877 GTCTTC GAAGAC(2/6)
BcgI 803 GCACTCAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 375 GTATCC GTATCC(6/5)
BpmI 533 CTGGAG CTGGAG(16/14)
BpuAI 877 GTCTTC GAAGAC(2/6)
BsaI 536 GAGACC GGTCTC(1/5)
BsmBI 272 GAGACG CGTCTC(1/5)
BsmFI 908 GGGAC GGGAC(10/14)
BspDI 356 ATCGAT AT^CGAT
BspHI 972 TCATGA T^CATGA
BspMI 657 GCAGGT ACCTGC(4/8)
BstF5I 953 CATCC GGATG(2/0)
Cac8I 42 GCTGGC GC[ACGT]^[ACGT]GC
ClaI 356 ATCGAT AT^CGAT
EaeI 431 TGGCCA [CT]^GGCC[GA]
EarI 634 CTCTTC CTCTTC(1/4)
FokI 953 CATCC GGATG(9/13)
Ksp632I 634 CTCTTC CTCTTC(1/4)
MluNI 431 TGGCCA TGG^CCA
MscI 431 TGGCCA TGG^CCA
PleI 110 GAGTC GAGTC(4/5)
RcaI 972 TCATGA T^CATGA
RsaI 69 GTAC GT^AC
ScaI 68 AGTACT AGT^ACT
SspI 175 AATATT AAT^ATT
TaqI 357 TCGA T^CGA
TfiI 921 GAATC G^A[AT]TC
Tsp45I 835 GTGAC ^GT[GC]AC
Tsp509I 369 AATT ^AATT
TthHB8I 357 TCGA T^CGA
XmnI 388 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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Alw44I 219 GTGCAC G^TGCAC
801 GTGCAC
AlwI 8 GGATC GGATC(4/5)
764 GGATC
ApaLI 219 GTGCAC G^TGCAC
801 GTGCAC
BbvI 46 GCAGC GCAGC(8/12)
55 GCTGC
BsmI 21 GAATGC GAATGC(1/-1)
326 GCATTC
BssKI 60 CCTGG ^CC[ACGT]GG
777 CCTGG
BstAPI 16 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
49 GCAGAGGCTGC
BstNI 60 CCTGG CC^[AT]GG
777 CCTGG
EcoRII 60 CCTGG ^CC[AT]GG
777 CCTGG
EcoT14I 114 CCTTGG C^C[AT][AT]GG
428 CCTTGG
Fnu4HI 46 GCAGC GC^[ACGT]GC
55 GCTGC
HinfI 110 GAGTC G^A[ACGT]TC
921 GAATC
ItaI 46 GCAGC GC^[ACGT]GC
55 GCTGC
MaeII 275 ACGT A^CGT
746 ACGT
MslI 705 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
912 CATGCTGATG
MvaI 60 CCTGG CC^[AT]GG
777 CCTGG
NlaIV 505 GGCACC GG[ACGT]^[ACGT]CC
584 GGTTCC
NspI 563 GCATGT [GA]CATG^[CT]
911 ACATGC
ScrFI 60 CCTGG CC^[ACGT]GG
777 CCTGG
StyI 114 CCTTGG C^C[AT][AT]GG
428 CCTTGG
TseI 46 GCAGC G^C[AT]GC
55 GCTGC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AspHI BmyI BsaJI BsiHKAI BsmAI
Bsp1286I BsrI DdeI DpnII HaeIII
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII Sau3AI Tru9I
TspRI