Restriction map for
pFN21AE9462
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BfaI BfrI BglII
BlnI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstUI BstYI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MfeI MflI MluI
MroI MseI MunI MvnI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 921 GTATAC GT^[AC][GT]AC
AlwI 911 GGATC GGATC(4/5)
AocI 697 CCTCAGG CC^T[ACGT]AGG
Aor51HI 842 AGCGCT AGC^GCT
BanI 799 GGTGCC G^G[CT][GA]CC
BbsI 1366 GAAGAC GAAGAC(2/6)
BclI 659 TGATCA T^GATCA
BlpI 91 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 91 GCTCAGC GC^T[ACGT]AGC
BpuAI 1366 GAAGAC GAAGAC(2/6)
BsaAI 1447 TACGTG [CT]AC^GT[GA]
BsaBI 135 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 871 CTCCTC GAGGAG(10/8)
BsgI 367 GTGCAG GTGCAG(16/14)
BsrBI 267 CCGCTC CCGCTC(-3/-3)
BsrI 1235 CCAGT ACTGG(1/-1)
BssSI 442 CTCGTG CACGAG(-5/-1)
Bst1107I 921 GTATAC GTA^TAC
BstEII 1041 GGTGACC G^GT[ACGT]ACC
BstPI 1041 GGTGACC G^GT[ACGT]ACC
BstZ17I 921 GTATAC GTA^TAC
Bsu36I 697 CCTCAGG CC^T[ACGT]AGG
CelII 91 GCTCAGC GC^T[ACGT]AGC
Eco47III 842 AGCGCT AGC^GCT
Eco81I 697 CCTCAGG CC^T[ACGT]AGG
EcoNI 627 CCTGGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1041 GGTGACC G^GT[ACGT]ACC
FbaI 659 TGATCA T^GATCA
HincII 17 GTCAAC GT[CT]^[GA]AC
HindII 17 GTCAAC GT[CT]^[GA]AC
HinfI 85 GAGTC G^A[ACGT]TC
MamI 135 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 38 GCCGGC GCC^GGC
NcoI 1166 CCATGG C^CATGG
NdeI 897 CATATG CA^TATG
NgoMIV 38 GCCGGC G^CCGGC
PleI 85 GAGTC GAGTC(4/5)
PstI 225 CTGCAG CTGCA^G
PvuII 1225 CAGCTG CAG^CTG
SfcI 225 CTGCAG C^T[GA][CT]AG
TaqI 1351 TCGA T^CGA
TthHB8I 1351 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 113 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1225 CAGCTGCTG
ApaI 200 GGGCCC GGGCC^C
583 GGGCCC
AspHI 331 GTGCTC G[AT]GC[AT]^C
868 GTGCTC
AspI 757 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1044 GACCAAGTC
BanII 200 GGGCCC G[GA]GC[CT]^C
583 GGGCCC
BpmI 98 CTGGAG CTGGAG(16/14)
292 CTGGAG
BsaI 1108 GAGACC GGTCTC(1/5)
1032 GGTCTC
BsiHKAI 331 GTGCTC G[AT]GC[AT]^C
868 GTGCTC
BsmAI 1108 GAGAC GTCTC(1/5)
1033 GTCTC
BspLU11I 1079 ACATGT A^CATGT
1370 ACATGT
HaeII 491 GGCGCT [GA]GCGC^[CT]
842 AGCGCT
MspA1I 1209 CAGCGG C[AC]G^C[GT]G
1225 CAGCTG
PflFI 757 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1044 GACCAAGTC
SfaNI 1266 GATGC GCATC(5/9)
284 GCATC
SmaI 587 CCCGGG CCC^GGG
1091 CCCGGG
StuI 234 AGGCCT AGG^CCT
1157 AGGCCT
Tth111I 757 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1044 GACCAAGTC
XmaCI 587 CCCGGG C^CCGGG
1091 CCCGGG
XmaI 587 CCCGGG C^CCGGG
1091 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AflIII AluI AvaI
AvaII BalI BbvI BcnI BglI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PpuMI RsaI
Sau3AI Sau96I ScrFI StyI TseI
Tsp45I TspRI Van91I XcmI