Restriction map for
pFN21AE9460
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BlnI
BlpI BmyI Bpu1102I BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstF5I
BstPI BstUI BstXI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FauI
FbaI FokI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeI MluI MluNI MroI MscI
MvnI NaeI NarI NcoI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 735 GTATAC GT^[AC][GT]AC
AfaI 852 GTAC GT^AC
AlwI 841 GATCC GGATC(4/5)
AseI 160 ATTAAT AT^TAAT
AsnI 160 ATTAAT AT^TAAT
Asp700I 246 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 423 GGTCC G^G[AT]CC
BbsI 338 GTCTTC GAAGAC(2/6)
BbvCI 741 CCTCAGC CCTCAGC(-5/-2)
BbvI 356 GCTGC GCAGC(8/12)
BcnI 382 CCCGG CC^[GC]GG
BglI 796 GCCAAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 40 CTGGAG CTGGAG(16/14)
BpuAI 338 GTCTTC GAAGAC(2/6)
BsaAI 51 CACGTA [CT]AC^GT[GA]
BsaBI 453 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 913 GAGACC GGTCTC(1/5)
BsmI 871 GCATTC GAATGC(1/-1)
BsrBI 876 CCGCTC CCGCTC(-3/-3)
Bst1107I 735 GTATAC GTA^TAC
BstZ17I 735 GTATAC GTA^TAC
Cac8I 271 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 423 GGTCC G^G[ACGT]CC
DsaI 686 CCACGG C^C[GA][CT]GG
EarI 787 CTCTTC CTCTTC(1/4)
EcoRV 399 GATATC GAT^ATC
Fnu4HI 356 GCTGC GC^[ACGT]GC
HapII 383 CCGG C^CGG
HincII 217 GTTGAC GT[CT]^[GA]AC
HindII 217 GTTGAC GT[CT]^[GA]AC
HpaII 383 CCGG C^CGG
ItaI 356 GCTGC GC^[ACGT]GC
Ksp632I 787 CTCTTC CTCTTC(1/4)
MaeII 52 ACGT A^CGT
MamI 453 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 594 CAATTG C^AATTG
MslI 136 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 383 CCGG C^CGG
MunI 594 CAATTG C^AATTG
NciI 382 CCCGG CC^[GC]GG
PacI 157 TTAATTAA TTAAT^TAA
PshBI 160 ATTAAT AT^TAAT
RsaI 852 GTAC GT^AC
Sau96I 423 GGTCC G^G[ACGT]CC
SfaNI 149 GCATC GCATC(5/9)
SfcI 618 CTACAG C^T[GA][CT]AG
SspI 558 AATATT AAT^ATT
TseI 356 GCTGC G^C[AT]GC
XmnI 246 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 333 CCGC CCGC(-3/-1)
876 CCGC
BanI 231 GGCACC G^G[CT][GA]CC
438 GGTGCC
BglII 36 AGATCT A^GATCT
362 AGATCT
BsiYI 407 CCGTTGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
876 CCGCTCAAAGG
BslI 407 CCGTTGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
876 CCGCTCAAAGG
BsmAI 892 GAGAC GTCTC(1/5)
913 GAGAC
BsrI 25 CCAGT ACTGG(1/-1)
202 CCAGT
BstYI 36 AGATCT [GA]^GATC[CT]
362 AGATCT
DraI 502 TTTAAA TTT^AAA
564 TTTAAA
EcoT14I 235 CCTTGG C^C[AT][AT]GG
917 CCAAGG
HphI 858 TCACC GGTGA(8/7)
904 TCACC
MflI 36 AGATCT [GA]^GATC[CT]
362 AGATCT
NdeI 546 CATATG CA^TATG
699 CATATG
PshAI 331 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
750 GACAGAAGTC
PvuII 343 CAGCTG CAG^CTG
354 CAGCTG
StyI 235 CCTTGG C^C[AT][AT]GG
917 CCAAGG
TaqI 609 TCGA T^CGA
826 TCGA
Tsp45I 682 GTGAC ^GT[GC]AC
886 GTCAC
TthHB8I 609 TCGA T^CGA
826 TCGA
XhoII 36 AGATCT [GA]^GATC[CT]
362 AGATCT
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI BsaJI BssKI
BstNI DdeI DpnII EcoRII HinfI
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIV
Sau3AI ScrFI TfiI Tru9I Tsp509I
TspRI