Restriction map for
pFN21AE9455
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BbeI BbvCI BciVI
BfrI BglII BlnI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrFI BsrGI
BssHII BstBI BstEII BstPI BstXI
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SpeI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 969 CGCG CG^CG
AcyI 358 GACGCC G[GA]^CG[CT]C
AflIII 1199 ACATGT A^C[GA][CT]GT
Alw44I 1231 GTGCAC G^TGCAC
ApaLI 1231 GTGCAC G^TGCAC
AspI 1363 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1313 TGCGCA TGC^GCA
BalI 995 TGGCCA TGG^CCA
BamHI 507 GGATCC G^GATCC
BbrPI 490 CACGTG CAC^GTG
BcgI 770 GCATCTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 791 TGATCA T^GATCA
BfaI 1081 CTAG C^TAG
BsaBI 993 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 358 GACGCC G[GA]^CG[CT]C
BspLU11I 1199 ACATGT A^CATGT
BsrDI 913 GCAATG GCAATG(2/0)
BssSI 757 CTCGTG CACGAG(-5/-1)
Bst1107I 1138 GTATAC GTA^TAC
BstAPI 324 GCACGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 969 CGCG CG^CG
BstYI 507 GGATCC [GA]^GATC[CT]
BstZ17I 1138 GTATAC GTA^TAC
DraIII 1340 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1226 CCGTGG C^C[GA][CT]GG
FbaI 791 TGATCA T^GATCA
FspI 1313 TGCGCA TGC^GCA
HaeII 1299 GGCGCT [GA]GCGC^[CT]
HgaI 358 GACGC GACGC(5/10)
Hin1I 358 GACGCC G[GA]^CG[CT]C
HincII 140 GTCAAC GT[CT]^[GA]AC
HindII 140 GTCAAC GT[CT]^[GA]AC
HindIII 1069 AAGCTT A^AGCTT
MaeI 1081 CTAG C^TAG
MamI 993 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 507 GGATCC [GA]^GATC[CT]
MluNI 995 TGGCCA TGG^CCA
MscI 995 TGGCCA TGG^CCA
MvnI 969 CGCG CG^CG
NdeI 1206 CATATG CA^TATG
PflFI 1363 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 490 CACGTG CAC^GTG
PmlI 490 CACGTG CAC^GTG
PshAI 561 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1280 ACCTGGT A^CC[AT]GGT
SnaBI 744 TACGTA TAC^GTA
StuI 1556 AGGCCT AGG^CCT
Tth111I 1363 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 507 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 1036 GTCTAC GT^[AC][GT]AC
1138 GTATAC
AfaI 629 GTAC GT^AC
747 GTAC
AocI 501 CCTCAGG CC^T[ACGT]AGG
616 CCTGAGG
ApaI 470 GGGCCC GGGCC^C
1091 GGGCCC
BbsI 568 GTCTTC GAAGAC(2/6)
1162 GTCTTC
BglI 542 GCCTGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
614 GCCCTGAGGGC
BpmI 154 CTGGAG CTGGAG(16/14)
609 CTGGAG
BpuAI 568 GTCTTC GAAGAC(2/6)
1162 GTCTTC
BsaAI 490 CACGTG [CT]AC^GT[GA]
744 TACGTA
BsmI 51 GAATGC GAATGC(1/-1)
907 GCATTC
BspMI 857 ACCTGC ACCTGC(4/8)
102 GCAGGT
BsrBI 1524 CCGCTC CCGCTC(-3/-3)
1327 GAGCGG
BsrI 219 CCAGT ACTGG(1/-1)
269 CCAGT
Bsu36I 501 CCTCAGG CC^T[ACGT]AGG
616 CCTGAGG
EaeI 950 TGGCCG [CT]^GGCC[GA]
995 TGGCCA
EarI 1273 CTCTTC CTCTTC(1/4)
1459 CTCTTC
Eco81I 501 CCTCAGG CC^T[ACGT]AGG
616 CCTGAGG
EcoT14I 1412 CCTTGG C^C[AT][AT]GG
1541 CCAAGG
HinfI 480 GAGTC G^A[ACGT]TC
832 GACTC
Ksp632I 1273 CTCTTC CTCTTC(1/4)
1459 CTCTTC
MseI 838 TTAA T^TAA
1471 TTAA
PleI 832 GACTC GAGTC(4/5)
480 GAGTC
PstI 722 CTGCAG CTGCA^G
765 CTGCAG
RsaI 629 GTAC GT^AC
747 GTAC
SacI 120 GAGCTC GAGCT^C
1131 GAGCTC
SfcI 722 CTGCAG C^T[GA][CT]AG
765 CTGCAG
StyI 1412 CCTTGG C^C[AT][AT]GG
1541 CCAAGG
TaqI 1001 TCGA T^CGA
1166 TCGA
Tru9I 838 TTAA T^TAA
1471 TTAA
TthHB8I 1001 TCGA T^CGA
1166 TCGA
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BlpI BmyI Bpu1102I BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PvuII Sau3AI Sau96I
ScrFI SfaNI SmaI SphI TseI
Tsp45I TspRI Van91I XcmI XmaCI
XmaI