Restriction map for
pFN21AE9432
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# NO CUT ENZYMES ( 92 enzymes) #
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AccI AccII AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I EcoRI EcoT22I FseI
FspI HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfoI SfuI SgrAI
SnaBI SpeI SphI StuI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1225 GACGTC GACGT^C
Acc65I 2017 GGTACC G^GTACC
AcyI 1225 GACGTC G[GA]^CG[CT]C
Aor51HI 301 AGCGCT AGC^GCT
Asp700I 1964 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2017 GGTACC G^GTACC
AspI 1507 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 1440 TGATCA T^GATCA
BglII 1662 AGATCT A^GATCT
BlpI 1813 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1813 GCTCAGC GC^T[ACGT]AGC
BsaHI 1225 GACGTC G[GA]^CG[CT]C
BsmI 843 GCATTC GAATGC(1/-1)
Bsp1407I 895 TGTACA T^GTACA
BspLU11I 1905 ACATGT A^CATGT
BsrDI 939 GCAATG GCAATG(2/0)
BsrFI 2276 ACCGGC [GA]^CCGG[CT]
BsrGI 895 TGTACA T^GTACA
BstEII 1279 GGTCACC G^GT[ACGT]ACC
BstPI 1279 GGTCACC G^GT[ACGT]ACC
CelII 1813 GCTCAGC GC^T[ACGT]AGC
CfoI 302 GCGC GCG^C
Cfr10I 2276 ACCGGC [GA]^CCGG[CT]
DrdI 363 GACAAGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 301 AGCGCT AGC^GCT
EcoO65I 1279 GGTCACC G^GT[ACGT]ACC
EcoRV 1416 GATATC GAT^ATC
FbaI 1440 TGATCA T^GATCA
HaeII 301 AGCGCT [GA]GCGC^[CT]
HgaI 977 GACGC GACGC(5/10)
HhaI 302 GCGC GCG^C
Hin1I 1225 GACGTC G[GA]^CG[CT]C
HinP1I 302 GCGC G^CGC
HincII 790 GTCAAC GT[CT]^[GA]AC
HindII 790 GTCAAC GT[CT]^[GA]AC
HindIII 1011 AAGCTT A^AGCTT
KpnI 2017 GGTACC GGTAC^C
MseI 772 TTAA T^TAA
NdeI 1352 CATATG CA^TATG
NspI 1905 ACATGT [GA]CATG^[CT]
PflFI 1507 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 2306 GCTCTTC GCTCTTC(1/4)
SbfI 1113 CCTGCAGG CCTGCA^GG
SfiI 730 GGCCTGACTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1319 CCCGGG CCC^GGG
Sse8387I 1113 CCTGCAGG CCTGCA^GG
SspBI 895 TGTACA T^GTACA
SspI 989 AATATT AAT^ATT
TaqI 628 TCGA T^CGA
Tru9I 772 TTAA T^TAA
Tth111I 1507 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 628 TCGA T^CGA
XcmI 1017 CCATACCCAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1319 CCCGGG C^CCGGG
XmaI 1319 CCCGGG C^CCGGG
XmnI 1964 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 614 ACGTGT A^C[GA][CT]GT
1905 ACATGT
Alw44I 1170 GTGCAC G^TGCAC
1332 GTGCAC
AocI 1678 CCTGAGG CC^T[ACGT]AGG
2192 CCTCAGG
ApaLI 1170 GTGCAC G^TGCAC
1332 GTGCAC
BanI 715 GGTGCC G^G[CT][GA]CC
2017 GGTACC
BbsI 1964 GAAGAC GAAGAC(2/6)
2211 GTCTTC
BfaI 2063 CTAG C^TAG
2318 CTAG
BglI 718 GCCTACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
731 GCCTGACTGGC
BpuAI 1964 GAAGAC GAAGAC(2/6)
2211 GTCTTC
BsaI 63 GAGACC GGTCTC(1/5)
1505 GAGACC
BspHI 72 TCATGA T^CATGA
539 TCATGA
BspMI 485 ACCTGC ACCTGC(4/8)
1203 ACCTGC
Bsu36I 1678 CCTGAGG CC^T[ACGT]AGG
2192 CCTCAGG
DsaI 1070 CCATGG C^C[GA][CT]GG
1471 CCATGG
Eco81I 1678 CCTGAGG CC^T[ACGT]AGG
2192 CCTCAGG
EcoNI 1182 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1761 CCTCTCCAAGG
MaeI 2063 CTAG C^TAG
2318 CTAG
NcoI 1070 CCATGG C^CATGG
1471 CCATGG
PflMI 48 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1248 CCACACCCTGG
PpuMI 388 GGGACCT [GA]G^G[AT]CC[CT]
455 GGGTCCC
RcaI 72 TCATGA T^CATGA
539 TCATGA
SacI 339 GAGCTC GAGCT^C
2304 GAGCTC
SexAI 857 ACCTGGT A^CC[AT]GGT
2013 ACCAGGT
SmlI 1366 CTTGAG C^T[CT][GA]AG
2003 CTCAAG
Van91I 48 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1248 CCACACCCTGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I Tsp509I TspRI XhoII