Restriction map for
pFN21AE9421
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BcnI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AciI 980 GCGG CCGC(-3/-1)
AflII 990 CTTAAG C^TTAAG
AflIII 325 ACATGT A^C[GA][CT]GT
AlwI 406 GATCC GGATC(4/5)
AlwNI 306 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 6 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 668 TGGCCA TGG^CCA
BbvCI 22 GCTGAGG CCTCAGC(-5/-2)
BfaI 683 CTAG C^TAG
BfrI 990 CTTAAG C^TTAAG
BsaBI 162 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 187 CTGCAC GTGCAG(16/14)
BsiYI 18 CCAAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 18 CCAAGCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 302 GGGAC GGGAC(10/14)
BspHI 158 TCATGA T^CATGA
BspLU11I 325 ACATGT A^CATGT
BsrDI 393 CATTGC GCAATG(2/0)
BstF5I 338 CATCC GGATG(2/0)
DrdI 996 GACTGATTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 635 CCATGG C^C[GA][CT]GG
EaeI 668 TGGCCA [CT]^GGCC[GA]
EcoT22I 453 ATGCAT ATGCA^T
FokI 338 CATCC GGATG(9/13)
HgaI 214 GCGTC GACGC(5/10)
HincII 1005 GTCAAC GT[CT]^[GA]AC
HindII 1005 GTCAAC GT[CT]^[GA]AC
HphI 3 GGTGA GGTGA(8/7)
MaeI 683 CTAG C^TAG
MaeII 50 ACGT A^CGT
MamI 162 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 668 TGGCCA TGG^CCA
MscI 668 TGGCCA TGG^CCA
MslI 249 CACTGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 635 CCATGG C^CATGG
NsiI 453 ATGCAT ATGCA^T
NspI 325 ACATGT [GA]CATG^[CT]
PleI 245 GAGTC GAGTC(4/5)
RcaI 158 TCATGA T^CATGA
SmlI 990 CTTAAG C^T[CT][GA]AG
XcmI 626 CCAATGTCGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 6 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcsI 316 AAATTC [GA]^AATT[CT]
852 GAATTT
AfaI 294 GTAC GT^AC
698 GTAC
ApoI 316 AAATTC [GA]^AATT[CT]
852 GAATTT
AvaII 88 GGTCC G^G[AT]CC
594 GGTCC
BseRI 720 CTCCTC GAGGAG(10/8)
863 CTCCTC
BsmAI 1036 GAGAC GTCTC(1/5)
805 GTCTC
BsrI 153 ACTGG ACTGG(1/-1)
590 ACTGG
BstXI 770 CCAAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
945 CCAATGGTTTGG
Cfr13I 88 GGTCC G^G[ACGT]CC
594 GGTCC
DraII 87 AGGTCCT [GA]G^G[ACGT]CC[CT]
593 GGGTCCT
EcoO109I 87 AGGTCCT [GA]G^G[ACGT]CC[CT]
593 GGGTCCT
HaeIII 222 GGCC GG^CC
669 GGCC
MseI 68 TTAA T^TAA
991 TTAA
PpuMI 87 AGGTCCT [GA]G^G[AT]CC[CT]
593 GGGTCCT
RsaI 294 GTAC GT^AC
698 GTAC
Sau96I 88 GGTCC G^G[ACGT]CC
594 GGTCC
TaqI 93 TCGA T^CGA
496 TCGA
Tru9I 68 TTAA T^TAA
991 TTAA
Tsp45I 247 GTCAC ^GT[GC]AC
481 GTCAC
TthHB8I 93 TCGA T^CGA
496 TCGA
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstNI
Cac8I DdeI DpnII EcoRII EcoT14I
Fnu4HI HinfI ItaI MaeIII MboI
MboII MnlI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI ScrFI SfaNI
StyI TfiI TseI Tsp509I TspRI