Restriction map for
pFN21AE9403
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI BamHI BbrPI BcgI
BclI BfrI BglII BpmI BsaAI
BsaBI BsaI BseAI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI HincII HindII
HindIII HinfI HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 1399 GTAC GT^AC
AlwI 444 GGATC GGATC(4/5)
AlwNI 682 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 121 GTGCTC G[AT]GC[AT]^C
AviII 980 TGCGCA TGC^GCA
AvrII 1191 CCTAGG C^CTAGG
BalI 1370 TGGCCA TGG^CCA
BbsI 1012 GTCTTC GAAGAC(2/6)
BbvCI 1101 CCTCAGC CCTCAGC(-5/-2)
BciVI 1470 GGATAC GTATCC(6/5)
BlnI 1191 CCTAGG C^CTAGG
BlpI 597 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 597 GCTCAGC GC^T[ACGT]AGC
BpuAI 1012 GTCTTC GAAGAC(2/6)
BsiHKAI 121 GTGCTC G[AT]GC[AT]^C
BspHI 1004 TCATGA T^CATGA
BspMI 857 ACCTGC ACCTGC(4/8)
BsrBI 194 CCGCTC CCGCTC(-3/-3)
BssSI 573 CTCGTG CACGAG(-5/-1)
BstBI 1289 TTCGAA TT^CGAA
BstYI 444 GGATCT [GA]^GATC[CT]
CelII 597 GCTCAGC GC^T[ACGT]AGC
EcoNI 1319 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 78 GGCCGGCC GGCCGG^CC
FspI 980 TGCGCA TGC^GCA
MflI 444 GGATCT [GA]^GATC[CT]
MluNI 1370 TGGCCA TGG^CCA
MscI 1370 TGGCCA TGG^CCA
MslI 1179 CATTTCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 79 GCCGGC GCC^GGC
NgoMIV 79 GCCGGC G^CCGGC
NspV 1289 TTCGAA TT^CGAA
RcaI 1004 TCATGA T^CATGA
RsaI 1399 GTAC GT^AC
SfuI 1289 TTCGAA TT^CGAA
XcmI 593 CCACGCTCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 444 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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BsaWI 791 ACCGGA [AT]^CCGG[AT]
1217 ACCGGA
BsrI 45 ACTGG ACTGG(1/-1)
1393 CCAGT
BssHII 674 GCGCGC G^CGCGC
952 GCGCGC
BstXI 1304 CCAACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1349 CCAGCGGAGTGG
EarI 490 CTCTTC CTCTTC(1/4)
1335 CTCTTC
HgaI 169 GACGC GACGC(5/10)
1234 GACGC
Ksp632I 490 CTCTTC CTCTTC(1/4)
1335 CTCTTC
MaeII 923 ACGT A^CGT
1367 ACGT
NotI 258 GCGGCCGC GC^GGCCGC
642 GCGGCCGC
PstI 302 CTGCAG CTGCA^G
679 CTGCAG
SfaNI 1185 GATGC GCATC(5/9)
584 GCATC
SfcI 302 CTGCAG C^T[GA][CT]AG
679 CTGCAG
TaqI 1183 TCGA T^CGA
1290 TCGA
TspRI 305 CAGTG CA[GC]TG[ACGT][ACGT]^
1439 CAGTG
TthHB8I 1183 TCGA T^CGA
1290 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HpaII HphI
ItaI KasI KspI MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PvuII SacII
Sau3AI Sau96I ScrFI SfiI SfoI
SmaI StyI TseI Tsp45I XmaCI
XmaI