Restriction map for
pFN21AB8971
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
Alw44I AlwI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BclI BfaI
BfrI BglI BglII BlnI BpuAI
BsaBI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I ClaI CpoI DraI DraIII
DrdI EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
MaeI MamI MfeI MflI MluI
MroI MseI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsaI RsrII SacI SalI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI TfiI Tru9I Tsp509I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 661 GGCGTC G[GA]^CG[CT]C
AhdI 1062 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 955 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 992 CCTCAGG CC^T[ACGT]AGG
Aor51HI 432 AGCGCT AGC^GCT
ApaI 715 GGGCCC GGGCC^C
AspEI 1062 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 566 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 807 GGATAC GTATCC(6/5)
BsaAI 415 TACGTG [CT]AC^GT[GA]
BsaHI 661 GGCGTC G[GA]^CG[CT]C
BsrBI 835 GAGCGG CCGCTC(-3/-3)
BsrDI 173 GCAATG GCAATG(2/0)
BstEII 879 GGTGACC G^GT[ACGT]ACC
BstPI 879 GGTGACC G^GT[ACGT]ACC
Bsu36I 992 CCTCAGG CC^T[ACGT]AGG
DpnII 817 GATC ^GATC
EagI 465 CGGCCG C^GGCCG
Eam1105I 1062 GACCCACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 465 CGGCCG C^GGCCG
Eco47III 432 AGCGCT AGC^GCT
Eco52I 465 CGGCCG C^GGCCG
Eco81I 992 CCTCAGG CC^T[ACGT]AGG
EcoO65I 879 GGTGACC G^GT[ACGT]ACC
Hin1I 661 GGCGTC G[GA]^CG[CT]C
MaeII 416 ACGT A^CGT
MboI 817 GATC ^GATC
NdeII 817 GATC ^GATC
NlaIII 666 CATG CATG^
PflFI 566 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 956 AGGACCT [GA]G^G[AT]CC[CT]
PstI 775 CTGCAG CTGCA^G
Sau3AI 817 GATC ^GATC
SgrAI 724 CGCCGGCG C[GA]^CCGG[CT]G
StuI 110 AGGCCT AGG^CCT
Tth111I 566 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaI 586 CCCGAG C^[CT]CG[GA]G
718 CCCGAG
AvaII 183 GGTCC G^G[AT]CC
957 GGACC
BalI 188 TGGCCA TGG^CCA
419 TGGCCA
BanII 302 GGGCTC G[GA]GC[CT]^C
715 GGGCCC
BcgI 214 GCAGCGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
860 GCAAGGATGTCG
BlpI 70 GCTGAGC GC^T[ACGT]AGC
1046 GCTCAGC
BmyI 302 GGGCTC G[AGT]GC[ACT]^C
715 GGGCCC
BpmI 244 CTCCAG CTGGAG(16/14)
315 CTGGAG
Bpu1102I 70 GCTGAGC GC^T[ACGT]AGC
1046 GCTCAGC
BsaI 1060 GAGACC GGTCTC(1/5)
480 GGTCTC
BsiEI 408 CGACCG CG[GA][CT]^CG
465 CGGCCG
BsmFI 4 GGGAC GGGAC(10/14)
1079 GGGAC
BsoBI 586 CCCGAG C^[CT]CG[GA]G
718 CCCGAG
Bsp1286I 302 GGGCTC G[AGT]GC[ACT]^C
715 GGGCCC
BsrI 822 ACTGG ACTGG(1/-1)
134 CCAGT
BssHII 217 GCGCGC G^CGCGC
829 GCGCGC
CelII 70 GCTGAGC GC^T[ACGT]AGC
1046 GCTCAGC
HinfI 279 GACTC G^A[ACGT]TC
318 GAGTC
HphI 606 GGTGA GGTGA(8/7)
879 GGTGA
KspI 448 CCGCGG CCGC^GG
1011 CCGCGG
MaeIII 504 GTCAC ^GT[ACGT]AC
880 GTGAC
MluNI 188 TGGCCA TGG^CCA
419 TGGCCA
MscI 188 TGGCCA TGG^CCA
419 TGGCCA
PleI 279 GACTC GAGTC(4/5)
318 GAGTC
PvuII 247 CAGCTG CAG^CTG
778 CAGCTG
SacII 448 CCGCGG CCGC^GG
1011 CCGCGG
SapI 304 GCTCTTC GCTCTTC(1/4)
531 GCTCTTC
SfcI 775 CTGCAG C^T[GA][CT]AG
786 CTACAG
Tsp45I 504 GTCAC ^GT[GC]AC
880 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BsmAI BspMI
BsrFI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NgoMIV NlaIV
Sau96I ScrFI TseI TspRI