Restriction map for
pFN21AE1297
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BspDI BspEI BspLU11I
BsrDI BsrI BssHII BssSI BstAPI
BstEII BstF5I BstPI BstXI Bsu36I
CelII ClaI CpoI DraI DraII
DrdI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MslI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 118 GTATAC GT^[AC][GT]AC
AflIII 122 ACGTGT A^C[GA][CT]GT
AluI 109 AGCT AG^CT
AlwI 282 GATCC GGATC(4/5)
Asp700I 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 310 CCCGGG C^[CT]CG[GA]G
AvaII 144 GGTCC G^G[AT]CC
BalI 320 TGGCCA TGG^CCA
BanI 343 GGCACC G^G[CT][GA]CC
BanII 39 GGGCTC G[GA]GC[CT]^C
BpmI 273 CTCCAG CTGGAG(16/14)
BsaAI 121 TACGTG [CT]AC^GT[GA]
BseRI 165 CTCCTC GAGGAG(10/8)
BsiEI 290 CGGCCG CG[GA][CT]^CG
BsoBI 310 CCCGGG C^[CT]CG[GA]G
Bsp1407I 125 TGTACA T^GTACA
BspHI 185 TCATGA T^CATGA
BspMI 141 GCAGGT ACCTGC(4/8)
BsrBI 20 CCGCTC CCGCTC(-3/-3)
BsrFI 157 ACCGGC [GA]^CCGG[CT]
BsrGI 125 TGTACA T^GTACA
Bst1107I 118 GTATAC GTA^TAC
BstBI 211 TTCGAA TT^CGAA
BstYI 281 AGATCC [GA]^GATC[CT]
BstZ17I 118 GTATAC GTA^TAC
Cfr10I 157 ACCGGC [GA]^CCGG[CT]
Cfr13I 144 GGTCC G^G[ACGT]CC
DpnII 282 GATC ^GATC
DraIII 328 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 176 CCATGG C^C[GA][CT]GG
EagI 290 CGGCCG C^GGCCG
EclXI 290 CGGCCG C^GGCCG
Eco52I 290 CGGCCG C^GGCCG
HincII 199 GTCAAC GT[CT]^[GA]AC
HindII 199 GTCAAC GT[CT]^[GA]AC
HinfI 182 GAATC G^A[ACGT]TC
MaeII 122 ACGT A^CGT
MboI 282 GATC ^GATC
MflI 281 AGATCC [GA]^GATC[CT]
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
NcoI 176 CCATGG C^CATGG
NdeII 282 GATC ^GATC
NlaIV 343 GGCACC GG[ACGT]^[ACGT]CC
NspV 211 TTCGAA TT^CGAA
RcaI 185 TCATGA T^CATGA
Sau3AI 282 GATC ^GATC
Sau96I 144 GGTCC G^G[ACGT]CC
SfuI 211 TTCGAA TT^CGAA
SmaI 310 CCCGGG CCC^GGG
SspBI 125 TGTACA T^GTACA
TfiI 182 GAATC G^A[AT]TC
XhoII 281 AGATCC [GA]^GATC[CT]
XmaCI 310 CCCGGG C^CCGGG
XmaI 310 CCCGGG C^CCGGG
XmnI 189 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 27 CGCG CG^CG
222 CGCG
AfaI 126 GTAC GT^AC
363 GTAC
Aor51HI 257 AGCGCT AGC^GCT
370 AGCGCT
BcnI 310 CCCGG CC^[GC]GG
311 CCGGG
BmyI 39 GGGCTC G[AGT]GC[ACT]^C
342 GGGCAC
BsiYI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCGACACCGG
BslI 31 CCCAAGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
151 CCCGACACCGG
Bsp1286I 39 GGGCTC G[AGT]GC[ACT]^C
342 GGGCAC
BstNI 240 CCTGG CC^[AT]GG
275 CCAGG
BstUI 27 CGCG CG^CG
222 CGCG
EaeI 290 CGGCCG [CT]^GGCC[GA]
320 TGGCCA
Eco47III 257 AGCGCT AGC^GCT
370 AGCGCT
EcoRII 240 CCTGG ^CC[AT]GG
275 CCAGG
EcoT14I 176 CCATGG C^C[AT][AT]GG
347 CCAAGG
HaeII 257 AGCGCT [GA]GCGC^[CT]
370 AGCGCT
HapII 158 CCGG C^CGG
311 CCGG
HpaII 158 CCGG C^CGG
311 CCGG
MaeIII 55 GTGAC ^GT[ACGT]AC
355 GTCAC
MspI 158 CCGG C^CGG
311 CCGG
MvaI 240 CCTGG CC^[AT]GG
275 CCAGG
MvnI 27 CGCG CG^CG
222 CGCG
NciI 310 CCCGG CC^[GC]GG
311 CCGGG
NlaIII 177 CATG CATG^
186 CATG
RsaI 126 GTAC GT^AC
363 GTAC
SfaNI 161 GCATC GCATC(5/9)
218 GCATC
StyI 176 CCATGG C^C[AT][AT]GG
347 CCAAGG
Tsp45I 55 GTGAC ^GT[GC]AC
355 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BbvI BsaJI BssKI Cac8I
CfoI DdeI FauI Fnu4HI HaeIII
HhaI HinP1I HphI ItaI MboII
MnlI MwoI ScrFI TaqI TseI
TthHB8I