Restriction map for
pFN21AB5610
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BfrI BglI
BlnI BpmI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BssHII Bst1107I BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoT22I FseI
FspI Hin1I HpaI KasI KpnI
MluI MluNI MroI MscI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SapI SbfI SexAI
SfiI SfoI SfuI SmaI SpeI
Sse8387I SspI StuI SwaI XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1742 GTCGAC GT^[AC][GT]AC
AflIII 761 ACATGT A^C[GA][CT]GT
Alw44I 2083 GTGCAC G^TGCAC
AlwNI 1682 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2083 GTGCAC G^TGCAC
AseI 835 ATTAAT AT^TAAT
AsnI 835 ATTAAT AT^TAAT
AspI 1777 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 2103 GGATAC GTATCC(6/5)
BlpI 1903 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1903 GCTGAGC GC^T[ACGT]AGC
BsaAI 2674 TACGTA [CT]AC^GT[GA]
BsaBI 1813 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 924 GGTCTC GGTCTC(1/5)
BseRI 2216 GAGGAG GAGGAG(10/8)
BsmI 2843 GCATTC GAATGC(1/-1)
Bsp1407I 2936 TGTACA T^GTACA
BspHI 3006 TCATGA T^CATGA
BspLU11I 761 ACATGT A^CATGT
BsrBI 2536 GAGCGG CCGCTC(-3/-3)
BsrDI 2359 GCAATG GCAATG(2/0)
BsrFI 284 GCCGGT [GA]^CCGG[CT]
BsrGI 2936 TGTACA T^GTACA
BssSI 199 CTCGTG CACGAG(-5/-1)
BstAPI 1057 GCAATTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2820 GGTTACC G^GT[ACGT]ACC
BstPI 2820 GGTTACC G^GT[ACGT]ACC
CelII 1903 GCTGAGC GC^T[ACGT]AGC
Cfr10I 284 GCCGGT [GA]^CCGG[CT]
EaeI 2965 CGGCCA [CT]^GGCC[GA]
EarI 877 CTCTTC CTCTTC(1/4)
EcoO65I 2820 GGTTACC G^GT[ACGT]ACC
HaeII 2853 AGCGCC [GA]GCGC^[CT]
HgaI 178 GCGTC GACGC(5/10)
HincII 1742 GTCGAC GT[CT]^[GA]AC
HindII 1742 GTCGAC GT[CT]^[GA]AC
HindIII 1188 AAGCTT A^AGCTT
Ksp632I 877 CTCTTC CTCTTC(1/4)
MamI 1813 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1070 CATATG CA^TATG
PaeR7I 2223 CTCGAG C^TCGAG
PflFI 1777 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 835 ATTAAT AT^TAAT
PvuII 1682 CAGCTG CAG^CTG
RcaI 3006 TCATGA T^CATGA
SalI 1742 GTCGAC G^TCGAC
SgrAI 283 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 2674 TACGTA TAC^GTA
SphI 1178 GCATGC GCATG^C
SspBI 2936 TGTACA T^GTACA
Tth111I 1777 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2223 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BanI 287 GGTGCC G^G[CT][GA]CC
1891 GGTGCC
BanII 206 GAGCTC G[GA]GC[CT]^C
1906 GAGCTC
BcnI 185 CCCGG CC^[GC]GG
2525 CCGGG
BsgI 617 CTGCAC GTGCAG(16/14)
2445 CTGCAC
DraI 1341 TTTAAA TTT^AAA
2953 TTTAAA
DraII 254 AGGTCCT [GA]G^G[ACGT]CC[CT]
1531 AGGTCCC
EcoO109I 254 AGGTCCT [GA]G^G[ACGT]CC[CT]
1531 AGGTCCC
EcoRV 1235 GATATC GAT^ATC
2800 GATATC
EcoT14I 332 CCAAGG C^C[AT][AT]GG
2028 CCTTGG
KspI 13 CCGCGG CCGC^GG
39 CCGCGG
NciI 185 CCCGG CC^[GC]GG
2525 CCGGG
PflMI 909 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1186 CCAAGCTTTGG
PpuMI 254 AGGTCCT [GA]G^G[AT]CC[CT]
1531 AGGTCCC
PstI 1927 CTGCAG CTGCA^G
2201 CTGCAG
SacI 206 GAGCTC GAGCT^C
1906 GAGCTC
SacII 13 CCGCGG CCGC^GG
39 CCGCGG
ScaI 119 AGTACT AGT^ACT
1437 AGTACT
StyI 332 CCAAGG C^C[AT][AT]GG
2028 CCTTGG
Van91I 909 CCAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1186 CCAAGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BbsI BbvI BclI BfaI BglII
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII FauI
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MfeI MflI MnlI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII