Restriction map for
pFN21AE1177
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspHI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmBI Bsp1407I
BspEI BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I FauI FspI HindIII
HpaI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 720 GGTACC G^GTACC
AccI 407 GTCTAC GT^[AC][GT]AC
AcyI 144 GGCGCC G[GA]^CG[CT]C
AocI 61 CCTGAGG CC^T[ACGT]AGG
ApaI 1743 GGGCCC GGGCC^C
Asp700I 1456 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 720 GGTACC G^GTACC
AvaI 129 CCCGGG C^[CT]CG[GA]G
BanII 1743 GGGCCC G[GA]GC[CT]^C
BbeI 144 GGCGCC GGCGC^C
BbsI 817 GAAGAC GAAGAC(2/6)
BclI 165 TGATCA T^GATCA
BfaI 832 CTAG C^TAG
BlpI 974 GCTCAGC GC^T[ACGT]AGC
BmyI 1743 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 974 GCTCAGC GC^T[ACGT]AGC
BpuAI 817 GAAGAC GAAGAC(2/6)
BsaHI 144 GGCGCC G[GA]^CG[CT]C
BseRI 1207 GAGGAG GAGGAG(10/8)
BsgI 1156 GTGCAG GTGCAG(16/14)
BsmFI 453 GGGAC GGGAC(10/14)
BsmI 349 GAATGC GAATGC(1/-1)
BsoBI 129 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1743 GGGCCC G[AGT]GC[ACT]^C
BspDI 1687 ATCGAT AT^CGAT
BspHI 1383 TCATGA T^CATGA
BspLU11I 594 ACATGT A^CATGT
BspMI 609 GCAGGT ACCTGC(4/8)
BsrDI 353 GCAATG GCAATG(2/0)
BssSI 1286 CACGAG CACGAG(-5/-1)
BstYI 316 GGATCT [GA]^GATC[CT]
Bsu36I 61 CCTGAGG CC^T[ACGT]AGG
CelII 974 GCTCAGC GC^T[ACGT]AGC
ClaI 1687 ATCGAT AT^CGAT
DsaI 273 CCATGG C^C[GA][CT]GG
Eco81I 61 CCTGAGG CC^T[ACGT]AGG
EcoRI 542 GAATTC G^AATTC
EcoRV 794 GATATC GAT^ATC
FbaI 165 TGATCA T^GATCA
FseI 1219 GGCCGGCC GGCCGG^CC
HaeII 144 GGCGCC [GA]GCGC^[CT]
HgaI 435 GACGC GACGC(5/10)
Hin1I 144 GGCGCC G[GA]^CG[CT]C
KasI 144 GGCGCC G^GCGCC
KpnI 720 GGTACC GGTAC^C
MaeI 832 CTAG C^TAG
MflI 316 GGATCT [GA]^GATC[CT]
NarI 144 GGCGCC GG^CGCC
NcoI 273 CCATGG C^CATGG
PvuI 1467 CGATCG CGAT^CG
RcaI 1383 TCATGA T^CATGA
SfoI 144 GGCGCC GGC^GCC
SgrAI 140 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 129 CCCGGG CCC^GGG
SmlI 1015 CTTGAG C^T[CT][GA]AG
SspI 649 AATATT AAT^ATT
XhoII 316 GGATCT [GA]^GATC[CT]
XmaCI 129 CCCGGG C^CCGGG
XmaI 129 CCCGGG C^CCGGG
XmnI 1456 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 594 ACATGT A^C[GA][CT]GT
1480 ACGTGT
AlwNI 335 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1133 CAGGAACTG
AvaII 178 GGACC G^G[AT]CC
396 GGTCC
BglI 136 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
245 GCCTCCCTGGC
BpmI 182 CTGGAG CTGGAG(16/14)
320 CTGGAG
BsmAI 1018 GAGAC GTCTC(1/5)
266 GTCTC
BsrI 153 CCAGT ACTGG(1/-1)
1245 CCAGT
DraII 65 AGGCCCC [GA]G^G[ACGT]CC[CT]
1742 AGGGCCC
EcoO109I 65 AGGCCCC [GA]G^G[ACGT]CC[CT]
1742 AGGGCCC
EcoT14I 273 CCATGG C^C[AT][AT]GG
464 CCTTGG
EcoT22I 222 ATGCAT ATGCA^T
1121 ATGCAT
MslI 670 CATTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1759 CACGACAATG
MspA1I 79 CAGCTG C[AC]G^C[GT]G
1720 CAGCTG
NaeI 141 GCCGGC GCC^GGC
1220 GCCGGC
NgoMIV 141 GCCGGC G^CCGGC
1220 GCCGGC
NsiI 222 ATGCAT ATGCA^T
1121 ATGCAT
PleI 565 GACTC GAGTC(4/5)
712 GACTC
PstI 381 CTGCAG CTGCA^G
1717 CTGCAG
PvuII 79 CAGCTG CAG^CTG
1720 CAGCTG
StyI 273 CCATGG C^C[AT][AT]GG
464 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BanI BbvI BcnI
BsaJI BsiEI BsiYI BslI BsrFI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I