Restriction map for
pFN21AE5102
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# NO CUT ENZYMES ( 187 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MamI MboII
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccI 103 GTCTAC GT^[AC][GT]AC
AcsI 364 GAATTC [GA]^AATT[CT]
Alw44I 4 GTGCAC G^TGCAC
ApaLI 4 GTGCAC G^TGCAC
ApoI 364 GAATTC [GA]^AATT[CT]
AvaII 113 GGACC G^G[AT]CC
BamHI 297 GGATCC G^GATCC
BbrPI 292 CACGTG CAC^GTG
BsaAI 292 CACGTG [CT]AC^GT[GA]
BseRI 19 GAGGAG GAGGAG(10/8)
BsiYI 83 CCCTGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 83 CCCTGGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsrI 376 CCAGT ACTGG(1/-1)
BstF5I 63 GGATG GGATG(2/0)
BstXI 348 CCATCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 263 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 80 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 364 GAATTC G^AATTC
EcoT14I 109 CCTTGG C^C[AT][AT]GG
FokI 63 GGATG GGATG(9/13)
NlaIII 190 CATG CATG^
PmaCI 292 CACGTG CAC^GTG
PmlI 292 CACGTG CAC^GTG
SfaNI 157 GATGC GCATC(5/9)
SmlI 244 CTCAAG C^T[CT][GA]AG
StyI 109 CCTTGG C^C[AT][AT]GG
Tsp45I 281 GTGAC ^GT[GC]AC
Tsp509I 365 AATT ^AATT
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 272 AGCT AG^CT
287 AGCT
AocI 16 CCTGAGG CC^T[ACGT]AGG
175 CCTAAGG
AspHI 4 GTGCAC G[AT]GC[AT]^C
202 GTGCTC
BanI 208 GGTGCC G^G[CT][GA]CC
250 GGCACC
BsiHKAI 4 GTGCAC G[AT]GC[AT]^C
202 GTGCTC
BstYI 141 GGATCT [GA]^GATC[CT]
297 GGATCC
Bsu36I 16 CCTGAGG CC^T[ACGT]AGG
175 CCTAAGG
DpnII 142 GATC ^GATC
298 GATC
Eco81I 16 CCTGAGG CC^T[ACGT]AGG
175 CCTAAGG
HinfI 12 GACTC G^A[ACGT]TC
130 GAGTC
MaeIII 34 GTTAC ^GT[ACGT]AC
281 GTGAC
MboI 142 GATC ^GATC
298 GATC
MflI 141 GGATCT [GA]^GATC[CT]
297 GGATCC
MwoI 31 GCCGTTACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
406 GCTGGTGTGGC
NdeII 142 GATC ^GATC
298 GATC
PleI 12 GACTC GAGTC(4/5)
130 GAGTC
Sau3AI 142 GATC ^GATC
298 GATC
XhoII 141 GGATCT [GA]^GATC[CT]
297 GGATCC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AlwI BbvI BmyI BsaJI BsgI
Bsp1286I BssKI BstNI Cac8I Cfr13I
DdeI EcoRII Fnu4HI HaeIII HphI
ItaI MaeII MnlI MvaI NlaIV
Sau96I ScrFI TseI TspRI