Restriction map for
pFN21AB3278
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# NO CUT ENZYMES ( 73 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI AspEI AvrII
BamHI BciVI BfrI BlnI BsaI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BstAPI
BstBI BstEII BstPI Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoT22I FseI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tsp509I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1172 GGTACC G^GTACC
Asp700I 2343 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1172 GGTACC G^GTACC
BbrPI 1711 CACGTG CAC^GTG
BbvCI 2013 GCTGAGG CCTCAGC(-5/-2)
BcgI 672 CGAGGACGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1922 TGATCA T^GATCA
BglII 758 AGATCT A^GATCT
BsaBI 2490 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1034 ACCGGA [AT]^CCGG[AT]
BsgI 1305 CTGCAC GTGCAG(16/14)
BspHI 1058 TCATGA T^CATGA
Bst1107I 691 GTATAC GTA^TAC
BstZ17I 691 GTATAC GTA^TAC
EarI 958 CTCTTC CTCTTC(1/4)
EcoNI 885 CCTCGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2068 GATATC GAT^ATC
FbaI 1922 TGATCA T^GATCA
HindIII 1875 AAGCTT A^AGCTT
HpaI 2563 GTTAAC GTT^AAC
KpnI 1172 GGTACC GGTAC^C
Ksp632I 958 CTCTTC CTCTTC(1/4)
KspI 913 CCGCGG CCGC^GG
MamI 2490 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2564 TTAA T^TAA
NcoI 2709 CCATGG C^CATGG
NdeI 2113 CATATG CA^TATG
PmaCI 1711 CACGTG CAC^GTG
PmlI 1711 CACGTG CAC^GTG
PpuMI 239 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1058 TCATGA T^CATGA
SacII 913 CCGCGG CCGC^GG
SapI 957 GCTCTTC GCTCTTC(1/4)
SbfI 2583 CCTGCAGG CCTGCA^GG
SfiI 2148 GGCCATCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 647 CCCGGG CCC^GGG
Sse8387I 2583 CCTGCAGG CCTGCA^GG
Tru9I 2564 TTAA T^TAA
XcmI 1919 CCATGATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 647 CCCGGG C^CCGGG
XmaI 647 CCCGGG C^CCGGG
XmnI 2343 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 34 enzymes) #
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AlwI 1206 GATCC GGATC(4/5)
2480 GGATC
AlwNI 1606 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2295 CAGCCCCTG
ApaI 1312 GGGCCC GGGCC^C
2624 GGGCCC
AspI 1285 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1803 GACCCTGTC
AviII 413 TGCGCA TGC^GCA
2285 TGCGCA
BfaI 271 CTAG C^TAG
2702 CTAG
BsaAI 733 TACGTG [CT]AC^GT[GA]
1711 CACGTG
BsiEI 1758 CGGCCG CG[GA][CT]^CG
1975 CGGCCG
BsmBI 2313 CGTCTC CGTCTC(1/5)
541 GAGACG
BstXI 212 CCATCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2337 CCACGAGAATGG
BstYI 758 AGATCT [GA]^GATC[CT]
2480 GGATCT
EagI 1758 CGGCCG C^GGCCG
1975 CGGCCG
EclXI 1758 CGGCCG C^GGCCG
1975 CGGCCG
Eco52I 1758 CGGCCG C^GGCCG
1975 CGGCCG
EcoT14I 566 CCAAGG C^C[AT][AT]GG
2709 CCATGG
FspI 413 TGCGCA TGC^GCA
2285 TGCGCA
MaeI 271 CTAG C^TAG
2702 CTAG
MflI 758 AGATCT [GA]^GATC[CT]
2480 GGATCT
NaeI 331 GCCGGC GCC^GGC
1909 GCCGGC
NgoMIV 331 GCCGGC G^CCGGC
1909 GCCGGC
PaeR7I 886 CTCGAG C^TCGAG
1166 CTCGAG
PflFI 1285 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1803 GACCCTGTC
PflMI 443 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
935 CCAACATCTGG
PvuII 474 CAGCTG CAG^CTG
2545 CAGCTG
SacI 880 GAGCTC GAGCT^C
2058 GAGCTC
SexAI 1430 ACCTGGT A^CC[AT]GGT
2234 ACCTGGT
SphI 2178 GCATGC GCATG^C
2282 GCATGC
StuI 2216 AGGCCT AGG^CCT
2610 AGGCCT
StyI 566 CCAAGG C^C[AT][AT]GG
2709 CCATGG
TfiI 171 GATTC G^A[AT]TC
1635 GATTC
Tth111I 1285 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1803 GACCCTGTC
Van91I 443 CCAGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
935 CCAACATCTGG
XhoI 886 CTCGAG C^TCGAG
1166 CTCGAG
XhoII 758 AGATCT [GA]^GATC[CT]
2480 GGATCT
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# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI TaqI
TseI Tsp45I TspRI TthHB8I