Restriction map for
pFN21AE0359
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AvaI AvrII BalI BamHI
BbrPI BcgI BciVI BfrI BglII
BlnI BsaAI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EarI Eco47III EcoO65I EcoRV EcoT22I
FseI HgaI HincII HindII HpaI
Ksp632I MfeI MluI MluNI MroI
MscI MunI NaeI NgoMIV NheI
NotI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp45I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 107 GGTACC G^GTACC
AcyI 123 GGCGCC G[GA]^CG[CT]C
AflIII 533 ACATGT A^C[GA][CT]GT
AlwI 1483 GATCC GGATC(4/5)
AocI 24 CCTGAGG CC^T[ACGT]AGG
Asp700I 892 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 107 GGTACC G^GTACC
AspI 359 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1297 GGACC G^G[AT]CC
AviII 1394 TGCGCA TGC^GCA
BbeI 123 GGCGCC GGCGC^C
BbsI 1456 GAAGAC GAAGAC(2/6)
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BclI 1590 TGATCA T^GATCA
BlpI 1734 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1734 GCTCAGC GC^T[ACGT]AGC
BpuAI 1456 GAAGAC GAAGAC(2/6)
BsaBI 811 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 123 GGCGCC G[GA]^CG[CT]C
BsgI 1070 GTGCAG GTGCAG(16/14)
BsiEI 205 CGGCCG CG[GA][CT]^CG
BsmFI 164 GGGAC GGGAC(10/14)
BspHI 1452 TCATGA T^CATGA
BspLU11I 533 ACATGT A^CATGT
BsrFI 144 ACCGGC [GA]^CCGG[CT]
BstXI 879 CCATAAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1482 AGATCC [GA]^GATC[CT]
Bsu36I 24 CCTGAGG CC^T[ACGT]AGG
CelII 1734 GCTCAGC GC^T[ACGT]AGC
Cfr10I 144 ACCGGC [GA]^CCGG[CT]
DraII 448 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1412 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 205 CGGCCG C^GGCCG
EclXI 205 CGGCCG C^GGCCG
Eco52I 205 CGGCCG C^GGCCG
Eco81I 24 CCTGAGG CC^T[ACGT]AGG
EcoNI 901 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 448 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1540 GAATTC G^AATTC
FbaI 1590 TGATCA T^GATCA
FspI 1394 TGCGCA TGC^GCA
HaeII 123 GGCGCC [GA]GCGC^[CT]
Hin1I 123 GGCGCC G[GA]^CG[CT]C
HindIII 839 AAGCTT A^AGCTT
KasI 123 GGCGCC G^GCGCC
KpnI 107 GGTACC GGTAC^C
MamI 811 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1482 AGATCC [GA]^GATC[CT]
NarI 123 GGCGCC GG^CGCC
NcoI 281 CCATGG C^CATGG
NruI 50 TCGCGA TCG^CGA
PflFI 359 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1232 CTGCAG CTGCA^G
RcaI 1452 TCATGA T^CATGA
SexAI 1413 ACCTGGT A^CC[AT]GGT
SfcI 1232 CTGCAG C^T[GA][CT]AG
SfoI 123 GGCGCC GGC^GCC
SmlI 915 CTTGAG C^T[CT][GA]AG
Tth111I 359 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1482 AGATCC [GA]^GATC[CT]
XmnI 892 GAATACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 108 GTAC GT^AC
288 GTAC
AlwNI 635 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1547 CAGATTCTG
BanII 252 GAGCCC G[GA]GC[CT]^C
277 GAGCCC
BfaI 1046 CTAG C^TAG
1616 CTAG
BglI 115 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
212 GCCGCGGCGGC
BmyI 252 GAGCCC G[AGT]GC[ACT]^C
277 GAGCCC
BsmAI 911 GAGAC GTCTC(1/5)
1228 GAGAC
Bsp1286I 252 GAGCCC G[AGT]GC[ACT]^C
277 GAGCCC
BstNI 901 CCTGG CC^[AT]GG
1414 CCTGG
EaeI 205 CGGCCG [CT]^GGCC[GA]
269 TGGCCG
EcoRII 901 CCTGG ^CC[AT]GG
1414 CCTGG
EcoT14I 281 CCATGG C^C[AT][AT]GG
1682 CCAAGG
FauI 159 CCCGC CCCGC(4/6)
162 GCGGG
KspI 160 CCGCGG CCGC^GG
213 CCGCGG
MaeI 1046 CTAG C^TAG
1616 CTAG
MaeIII 737 GTTAC ^GT[ACGT]AC
1401 GTTAC
MvaI 901 CCTGG CC^[AT]GG
1414 CCTGG
NdeI 992 CATATG CA^TATG
1223 CATATG
NspI 533 ACATGT [GA]CATG^[CT]
1397 GCATGT
RsaI 108 GTAC GT^AC
288 GTAC
SacII 160 CCGCGG CCGC^GG
213 CCGCGG
StyI 281 CCATGG C^C[AT][AT]GG
1682 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
BanI BbvI BcnI BpmI BsaJI
BseRI BsiYI BslI BsrI BssKI
BstF5I BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MseI MslI MspA1I
MspI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I