Restriction map for
pFN21AB3014
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# NO CUT ENZYMES ( 110 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI Asp718I AspEI
AviII AvrII BbeI BbvCI BcgI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Alw44I 2820 GTGCAC G^TGCAC
ApaLI 2820 GTGCAC G^TGCAC
AseI 2587 ATTAAT AT^TAAT
AsnI 2587 ATTAAT AT^TAAT
BamHI 2123 GGATCC G^GATCC
BanI 1771 GGTGCC G^G[CT][GA]CC
BanII 1588 GAGCCC G[GA]GC[CT]^C
BbrPI 2321 CACGTG CAC^GTG
BciVI 1731 GTATCC GTATCC(6/5)
BcnI 1176 CCGGG CC^[GC]GG
BglII 3008 AGATCT A^GATCT
BsaAI 2321 CACGTG [CT]AC^GT[GA]
BsaWI 2800 ACCGGA [AT]^CCGG[AT]
BseRI 1914 CTCCTC GAGGAG(10/8)
BsmFI 1955 GGGAC GGGAC(10/14)
BspHI 2684 TCATGA T^CATGA
BspMI 2459 ACCTGC ACCTGC(4/8)
BssSI 2303 CACGAG CACGAG(-5/-1)
BstEII 1939 GGTGACC G^GT[ACGT]ACC
BstPI 1939 GGTGACC G^GT[ACGT]ACC
BstXI 2037 CCATATTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2207 GCGC GCG^C
DraI 1434 TTTAAA TTT^AAA
DsaI 1775 CCATGG C^C[GA][CT]GG
EcoO65I 1939 GGTGACC G^GT[ACGT]ACC
EcoRI 2690 GAATTC G^AATTC
HaeII 2206 GGCGCT [GA]GCGC^[CT]
HhaI 2207 GCGC GCG^C
HinP1I 2207 GCGC G^CGC
HindIII 1670 AAGCTT A^AGCTT
MslI 1858 CATAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 1176 CCGGG CC^[GC]GG
NcoI 1775 CCATGG C^CATGG
PmaCI 2321 CACGTG CAC^GTG
PmlI 2321 CACGTG CAC^GTG
PshBI 2587 ATTAAT AT^TAAT
RcaI 2684 TCATGA T^CATGA
SspI 2946 AATATT AAT^ATT
StuI 497 AGGCCT AGG^CCT
TaqI 2455 TCGA T^CGA
TthHB8I 2455 TCGA T^CGA
XbaI 858 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AspHI 1691 GTGCTC G[AT]GC[AT]^C
2820 GTGCAC
AspI 2635 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2662 GACATAGTC
AvaI 8 CCCGAG C^[CT]CG[GA]G
2483 CTCGGG
AvaII 217 GGACC G^G[AT]CC
648 GGACC
BalI 2180 TGGCCA TGG^CCA
2972 TGGCCA
BbsI 1853 GTCTTC GAAGAC(2/6)
2134 GTCTTC
BpuAI 1853 GTCTTC GAAGAC(2/6)
2134 GTCTTC
BsiHKAI 1691 GTGCTC G[AT]GC[AT]^C
2820 GTGCAC
BsmAI 1037 GAGAC GTCTC(1/5)
69 GTCTC
BsoBI 8 CCCGAG C^[CT]CG[GA]G
2483 CTCGGG
BspLU11I 1727 ACATGT A^CATGT
2019 ACATGT
EaeI 2180 TGGCCA [CT]^GGCC[GA]
2972 TGGCCA
EcoNI 682 CCTAACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2163 CCTCTCTGAGG
EcoRV 1327 GATATC GAT^ATC
2254 GATATC
HapII 1176 CCGG C^CGG
2801 CCGG
HpaII 1176 CCGG C^CGG
2801 CCGG
MaeII 2010 ACGT A^CGT
2322 ACGT
MfeI 557 CAATTG C^AATTG
2630 CAATTG
MluNI 2180 TGGCCA TGG^CCA
2972 TGGCCA
MscI 2180 TGGCCA TGG^CCA
2972 TGGCCA
MspI 1176 CCGG C^CGG
2801 CCGG
MunI 557 CAATTG C^AATTG
2630 CAATTG
PflFI 2635 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2662 GACATAGTC
SfaNI 2107 GATGC GCATC(5/9)
2851 GATGC
SmlI 1079 CTCAAG C^T[CT][GA]AG
1198 CTTGAG
SphI 782 GCATGC GCATG^C
2351 GCATGC
Tth111I 2635 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2662 GACATAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
BbvI BfaI BglI BmyI BpmI
BsaJI BsgI BsiYI BslI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII EarI
Eco81I EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI XhoII XmnI