Restriction map for
pFN21AE6160
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# NO CUT ENZYMES ( 182 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AluI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BbvI BcgI BciVI
BclI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FauI FbaI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI ItaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I TseI TspRI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 71 CCGC CCGC(-3/-1)
AcsI 317 AAATTC [GA]^AATT[CT]
AlwI 192 GATCC GGATC(4/5)
ApoI 317 AAATTC [GA]^AATT[CT]
BanII 220 GGGCTC G[GA]GC[CT]^C
BcnI 84 CCGGG CC^[GC]GG
BfaI 196 CTAG C^TAG
BglII 294 AGATCT A^GATCT
BmyI 220 GGGCTC G[AGT]GC[ACT]^C
BpmI 62 CTCCAG CTGGAG(16/14)
BsmBI 268 GAGACG CGTCTC(1/5)
Bsp1286I 220 GGGCTC G[AGT]GC[ACT]^C
BspMI 75 ACCTGC ACCTGC(4/8)
BstEII 157 GGTGACC G^GT[ACGT]ACC
BstF5I 68 CATCC GGATG(2/0)
BstPI 157 GGTGACC G^GT[ACGT]ACC
DraII 129 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 129 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 157 GGTGACC G^GT[ACGT]ACC
EcoT14I 323 CCAAGG C^C[AT][AT]GG
FokI 68 CATCC GGATG(9/13)
HaeIII 210 GGCC GG^CC
HapII 84 CCGG C^CGG
HinfI 60 GACTC G^A[ACGT]TC
HpaII 84 CCGG C^CGG
HphI 157 GGTGA GGTGA(8/7)
MaeI 196 CTAG C^TAG
MaeII 271 ACGT A^CGT
MaeIII 158 GTGAC ^GT[ACGT]AC
MboII 331 GAAGA GAAGA(8/7)
MspI 84 CCGG C^CGG
MwoI 302 GCATGCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 84 CCGGG CC^[GC]GG
NspI 302 GCATGC [GA]CATG^[CT]
PflMI 283 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 60 GACTC GAGTC(4/5)
PpuMI 129 GGGTCCC [GA]G^G[AT]CC[CT]
SfaNI 67 GCATC GCATC(5/9)
SmlI 187 CTTGAG C^T[CT][GA]AG
SphI 302 GCATGC GCATG^C
StyI 323 CCAAGG C^C[AT][AT]GG
TaqI 215 TCGA T^CGA
Tsp45I 158 GTGAC ^GT[GC]AC
Tsp509I 318 AATT ^AATT
TthHB8I 215 TCGA T^CGA
Van91I 283 CCAGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BlpI 45 GCTGAGC GC^T[ACGT]AGC
306 GCTCAGC
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
306 GCTCAGC
BstNI 21 CCTGG CC^[AT]GG
51 CCTGG
BstYI 191 AGATCC [GA]^GATC[CT]
294 AGATCT
Cac8I 115 GCTGGC GC[ACGT]^[ACGT]GC
302 GCATGC
CelII 45 GCTGAGC GC^T[ACGT]AGC
306 GCTCAGC
DdeI 46 CTGAG C^T[ACGT]AG
307 CTCAG
DpnII 192 GATC ^GATC
295 GATC
EcoNI 201 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
248 CCTCCAAGAGG
EcoRII 21 CCTGG ^CC[AT]GG
51 CCTGG
MboI 192 GATC ^GATC
295 GATC
MflI 191 AGATCC [GA]^GATC[CT]
294 AGATCT
MvaI 21 CCTGG CC^[AT]GG
51 CCTGG
NdeII 192 GATC ^GATC
295 GATC
NlaIII 303 CATG CATG^
312 CATG
Sau3AI 192 GATC ^GATC
295 GATC
XhoII 191 AGATCC [GA]^GATC[CT]
294 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AvaII BsaJI BseRI BsiYI BslI
BsmAI BsmFI BssKI Cfr13I MnlI
NlaIV Sau96I ScrFI