Restriction map for
pFN21AB6165
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# NO CUT ENZYMES ( 115 enzymes) #
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Acc65I AccI AccIII AclI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbeI BbrPI BcgI BclI
BfaI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MunI NarI NcoI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccII 360 CGCG CG^CG
AcsI 948 GAATTC [GA]^AATT[CT]
AflII 850 CTTAAG C^TTAAG
ApoI 948 GAATTC [GA]^AATT[CT]
AspHI 907 GAGCAC G[AT]GC[AT]^C
AviII 1112 TGCGCA TGC^GCA
BalI 1124 TGGCCA TGG^CCA
BbsI 400 GTCTTC GAAGAC(2/6)
BbvCI 1911 CCTCAGC CCTCAGC(-5/-2)
BciVI 294 GTATCC GTATCC(6/5)
BfrI 850 CTTAAG C^TTAAG
BglII 887 AGATCT A^GATCT
BpuAI 400 GTCTTC GAAGAC(2/6)
BsiHKAI 907 GAGCAC G[AT]GC[AT]^C
BsmFI 74 GGGAC GGGAC(10/14)
BstAPI 1022 GCAAGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 360 CGCG CG^CG
DraIII 1820 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 78 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 948 GAATTC G^AATTC
FspI 1112 TGCGCA TGC^GCA
HaeII 65 AGCGCC [GA]GCGC^[CT]
HgaI 211 GCGTC GACGC(5/10)
MluNI 1124 TGGCCA TGG^CCA
MscI 1124 TGGCCA TGG^CCA
MvnI 360 CGCG CG^CG
NdeI 664 CATATG CA^TATG
NspI 126 GCATGC [GA]CATG^[CT]
PleI 1468 GACTC GAGTC(4/5)
SapI 1135 GAAGAGC GCTCTTC(1/4)
ScaI 698 AGTACT AGT^ACT
SmaI 1797 CCCGGG CCC^GGG
SphI 126 GCATGC GCATG^C
TfiI 341 GATTC G^A[AT]TC
XmaCI 1797 CCCGGG C^CCGGG
XmaI 1797 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AatII 28 GACGTC GACGT^C
397 GACGTC
AcyI 28 GACGTC G[GA]^CG[CT]C
397 GACGTC
AlwI 480 GATCC GGATC(4/5)
1099 GATCC
AvaI 223 CCCGAG C^[CT]CG[GA]G
1797 CCCGGG
BsaHI 28 GACGTC G[GA]^CG[CT]C
397 GACGTC
BsaI 415 GAGACC GGTCTC(1/5)
1568 GAGACC
BsgI 1267 CTGCAC GTGCAG(16/14)
1831 CTGCAC
BsoBI 223 CCCGAG C^[CT]CG[GA]G
1797 CCCGGG
BspMI 1829 ACCTGC ACCTGC(4/8)
1780 GCAGGT
BsrDI 207 CATTGC GCAATG(2/0)
1104 CATTGC
BsrFI 130 GCCGGC [GA]^CCGG[CT]
250 GCCGGC
BstEII 1683 GGTGACC G^GT[ACGT]ACC
1825 GGTGACC
BstPI 1683 GGTGACC G^GT[ACGT]ACC
1825 GGTGACC
Cfr10I 130 GCCGGC [GA]^CCGG[CT]
250 GCCGGC
DsaI 1883 CCACGG C^C[GA][CT]GG
1931 CCGTGG
EcoO65I 1683 GGTGACC G^GT[ACGT]ACC
1825 GGTGACC
EcoT14I 69 CCAAGG C^C[AT][AT]GG
1572 CCTTGG
Hin1I 28 GACGTC G[GA]^CG[CT]C
397 GACGTC
HinfI 341 GATTC G^A[ACGT]TC
1468 GACTC
MseI 446 TTAA T^TAA
851 TTAA
MslI 837 CATCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1104 CATTGCTGTG
NaeI 130 GCCGGC GCC^GGC
250 GCCGGC
NgoMIV 130 GCCGGC G^CCGGC
250 GCCGGC
PpuMI 74 GGGACCT [GA]G^G[AT]CC[CT]
1319 AGGACCT
PstI 1315 CTGCAG CTGCA^G
1892 CTGCAG
PvuII 138 CAGCTG CAG^CTG
1247 CAGCTG
SbfI 1314 CCTGCAGG CCTGCA^GG
1891 CCTGCAGG
Sse8387I 1314 CCTGCAGG CCTGCA^GG
1891 CCTGCAGG
StyI 69 CCAAGG C^C[AT][AT]GG
1572 CCTTGG
Tru9I 446 TTAA T^TAA
851 TTAA
Tsp509I 895 AATT ^AATT
949 AATT
XcmI 725 CCATACCCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1226 CCATCCGGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII