Restriction map for
pFN21AE1104
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# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BciVI BclI
BcnI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HapII
HhaI HinP1I HindIII HinfI HpaI
HpaII KasI Ksp632I KspI MaeI
MaeII MamI MboI MfeI MflI
MluI MluNI MroI MscI MseI
MspI MunI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 275 GGTACC G^GTACC
AccII 343 CGCG CG^CG
AcyI 127 GACGCC G[GA]^CG[CT]C
AlwNI 331 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 418 CCTGAGG CC^T[ACGT]AGG
ApaI 51 GGGCCC GGGCC^C
Asp718I 275 GGTACC G^GTACC
AspHI 297 GAGCAC G[AT]GC[AT]^C
AvaII 288 GGTCC G^G[AT]CC
BanI 275 GGTACC G^G[CT][GA]CC
BcgI 144 GCAGCTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 127 GACGCC G[GA]^CG[CT]C
BsaI 312 GGTCTC GGTCTC(1/5)
BsiHKAI 297 GAGCAC G[AT]GC[AT]^C
BsmAI 313 GTCTC GTCTC(1/5)
BspMI 285 GCAGGT ACCTGC(4/8)
BstUI 343 CGCG CG^CG
Bsu36I 418 CCTGAGG CC^T[ACGT]AGG
Cac8I 210 GCTGGC GC[ACGT]^[ACGT]GC
DsaI 79 CCACGG C^C[GA][CT]GG
Eco81I 418 CCTGAGG CC^T[ACGT]AGG
FauI 161 CCCGC CCCGC(4/6)
Hin1I 127 GACGCC G[GA]^CG[CT]C
HincII 304 GTCAAC GT[CT]^[GA]AC
HindII 304 GTCAAC GT[CT]^[GA]AC
KpnI 275 GGTACC GGTAC^C
MaeIII 167 GTCAC ^GT[ACGT]AC
MboII 349 GAAGA GAAGA(8/7)
MslI 327 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 139 CAGCTG C[AC]G^C[GT]G
MvnI 343 CGCG CG^CG
PflMI 330 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 287 AGGTCCC [GA]G^G[AT]CC[CT]
PvuII 139 CAGCTG CAG^CTG
SmlI 223 CTCAAG C^T[CT][GA]AG
Tsp45I 167 GTCAC ^GT[GC]AC
TspRI 201 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 330 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 162 CCGC CCGC(-3/-1)
237 CCGC
AfaI 36 GTAC GT^AC
276 GTAC
AluI 140 AGCT AG^CT
146 AGCT
BanII 51 GGGCCC G[GA]GC[CT]^C
181 GAGCCC
BbvCI 270 GCTGAGG CCTCAGC(-5/-2)
424 GCTGAGG
BglI 205 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
236 GCCGCAAGGGC
DraII 227 AGGCCCT [GA]G^G[ACGT]CC[CT]
287 AGGTCCC
EcoNI 279 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
472 CCTGGTTCAGG
EcoO109I 227 AGGCCCT [GA]G^G[ACGT]CC[CT]
287 AGGTCCC
HgaI 127 GACGC GACGC(5/10)
267 GACGC
NlaIII 91 CATG CATG^
388 CATG
PstI 85 CTGCAG CTGCA^G
142 CTGCAG
RsaI 36 GTAC GT^AC
276 GTAC
SfcI 85 CTGCAG C^T[GA][CT]AG
142 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmFI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI EcoRII Fnu4HI
FokI HaeIII HphI ItaI MnlI
MvaI MwoI NlaIV Sau96I ScrFI
SfaNI TseI