Restriction map for
pFN21AB9715
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BlnI
BpmI BpuAI BsaAI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I ClaI DraI DraII
DraIII DrdI Eam1105I EarI Eco47III
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
MaeI MfeI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 835 ACACGT A^C[GA][CT]GT
AgeI 667 ACCGGT A^CCGGT
AlwI 846 GGATC GGATC(4/5)
AocI 330 CCTGAGG CC^T[ACGT]AGG
ApaI 775 GGGCCC GGGCC^C
AspHI 730 GAGCAC G[AT]GC[AT]^C
AspI 460 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 482 CCCGGG C^[CT]CG[GA]G
BglI 613 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1043 AGATCT A^GATCT
BlpI 761 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 761 GCTCAGC GC^T[ACGT]AGC
BsaBI 1038 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 458 GAGACC GGTCTC(1/5)
BsiHKAI 730 GAGCAC G[AT]GC[AT]^C
BsoBI 482 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1130 TGTACA T^GTACA
BspMI 451 GCAGGT ACCTGC(4/8)
BsrGI 1130 TGTACA T^GTACA
BssHII 1062 GCGCGC G^CGCGC
BstYI 1043 AGATCT [GA]^GATC[CT]
Bsu36I 330 CCTGAGG CC^T[ACGT]AGG
CelII 761 GCTCAGC GC^T[ACGT]AGC
Eco81I 330 CCTGAGG CC^T[ACGT]AGG
EcoNI 1005 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI 270 TCACC GGTGA(8/7)
MaeII 837 ACGT A^CGT
MaeIII 431 GTGAC ^GT[ACGT]AC
MamI 1038 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1043 AGATCT [GA]^GATC[CT]
NaeI 562 GCCGGC GCC^GGC
NgoMIV 562 GCCGGC G^CCGGC
NlaIII 1116 CATG CATG^
PflFI 460 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 468 CCACCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 667 ACCGGT A^CCGGT
PstI 943 CTGCAG CTGCA^G
SfcI 943 CTGCAG C^T[GA][CT]AG
SmaI 482 CCCGGG CCC^GGG
SmlI 952 CTCAAG C^T[CT][GA]AG
SspBI 1130 TGTACA T^GTACA
Tsp45I 431 GTGAC ^GT[GC]AC
Tth111I 460 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 468 CCACCCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1043 AGATCT [GA]^GATC[CT]
XmaCI 482 CCCGGG C^CCGGG
XmaI 482 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 828 GTAC GT^AC
1131 GTAC
BbvCI 78 CCTCAGC CCTCAGC(-5/-2)
247 CCTCAGC
BsaWI 667 ACCGGT [AT]^CCGG[AT]
859 TCCGGT
BstF5I 327 CATCC GGATG(2/0)
854 CATCC
CpoI 861 CGGTCCG CG^G[AT]CCG
919 CGGACCG
FokI 327 CATCC GGATG(9/13)
854 CATCC
HgaI 520 GACGC GACGC(5/10)
574 GCGTC
PvuII 292 CAGCTG CAG^CTG
940 CAGCTG
RsaI 828 GTAC GT^AC
1131 GTAC
RsrII 861 CGGTCCG CG^G[AT]CCG
919 CGGACCG
SfaNI 252 GCATC GCATC(5/9)
1088 GCATC
SgrAI 561 CGCCGGCG C[GA]^CCGG[CT]G
666 CACCGGTG
TaqI 320 TCGA T^CGA
425 TCGA
TspRI 623 CAGTG CA[GC]TG[ACGT][ACGT]^
681 CAGTG
TthHB8I 320 TCGA T^CGA
425 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaII BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsgI BsiEI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BssKI BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EclXI Eco52I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
KspI MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV NotI PleI
SacII Sau3AI Sau96I ScrFI SfoI
TseI