Restriction map for
pFN21AB5056
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BanI
BbeI BclI BfaI BfrI BglII
BlnI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI DsaI Eco47III EcoO65I EcoRV
EcoT22I FbaI FspI HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MroI MseI MslI MunI
NarI NcoI NdeI NheI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfcI
SfoI SfuI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 562 GACGTC GACGT^C
AccI 565 GTCTAC GT^[AC][GT]AC
AcsI 1093 GAATTC [GA]^AATT[CT]
AfaI 369 GTAC GT^AC
Alw44I 1509 GTGCAC G^TGCAC
AlwNI 210 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1509 GTGCAC G^TGCAC
ApoI 1093 GAATTC [GA]^AATT[CT]
AspI 1537 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1557 TGGCCA TGG^CCA
BcgI 443 GCAACATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1313 GTATCC GTATCC(6/5)
BlpI 1392 GCTCAGC GC^T[ACGT]AGC
BpmI 297 CTGGAG CTGGAG(16/14)
Bpu1102I 1392 GCTCAGC GC^T[ACGT]AGC
BsaBI 1275 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 955 GAGACC GGTCTC(1/5)
BseRI 424 GAGGAG GAGGAG(10/8)
BsmAI 955 GAGAC GTCTC(1/5)
BsmI 454 GCATTC GAATGC(1/-1)
BstAPI 1511 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1392 GCTCAGC GC^T[ACGT]AGC
EcoNI 1593 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1093 GAATTC G^AATTC
FseI 1046 GGCCGGCC GGCCGG^CC
HaeII 414 GGCGCT [GA]GCGC^[CT]
HincII 1156 GTCAAC GT[CT]^[GA]AC
HindII 1156 GTCAAC GT[CT]^[GA]AC
MamI 1275 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1557 TGGCCA TGG^CCA
MscI 1557 TGGCCA TGG^CCA
PflFI 1537 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 332 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 369 GTAC GT^AC
SexAI 383 ACCTGGT A^CC[AT]GGT
SfiI 741 GGCCGCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 849 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1117 CCCGGG CCC^GGG
SmlI 1594 CTCAAG C^T[CT][GA]AG
SphI 1205 GCATGC GCATG^C
Tth111I 1537 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1117 CCCGGG C^CCGGG
XmaI 1117 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 1552 ACATGT A^C[GA][CT]GT
1562 ACATGT
AhdI 382 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1479 GACCCCAGGTC
AlwI 168 GATCC GGATC(4/5)
1275 GATCC
AocI 421 CCTGAGG CC^T[ACGT]AGG
1069 CCTGAGG
AspEI 382 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1479 GACCCCAGGTC
BbrPI 1023 CACGTG CAC^GTG
1398 CACGTG
BbsI 481 GTCTTC GAAGAC(2/6)
925 GTCTTC
BbvCI 180 CCTCAGC CCTCAGC(-5/-2)
1179 CCTCAGC
BglI 742 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1184 GCCACAATGGC
BpuAI 481 GTCTTC GAAGAC(2/6)
925 GTCTTC
BsaAI 1023 CACGTG [CT]AC^GT[GA]
1398 CACGTG
BspLU11I 1552 ACATGT A^CATGT
1562 ACATGT
BspMI 827 ACCTGC ACCTGC(4/8)
1055 ACCTGC
BstXI 1292 CCACTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1358 CCAGTGCCCTGG
BstYI 167 AGATCC [GA]^GATC[CT]
1274 AGATCC
Bsu36I 421 CCTGAGG CC^T[ACGT]AGG
1069 CCTGAGG
DraIII 1020 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1528 CACGCGGTG
Eam1105I 382 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1479 GACCCCAGGTC
EarI 139 CTCTTC CTCTTC(1/4)
1246 CTCTTC
Eco81I 421 CCTGAGG CC^T[ACGT]AGG
1069 CCTGAGG
EcoT14I 155 CCAAGG C^C[AT][AT]GG
410 CCAAGG
Ksp632I 139 CTCTTC CTCTTC(1/4)
1246 CTCTTC
MflI 167 AGATCC [GA]^GATC[CT]
1274 AGATCC
NotI 627 GCGGCCGC GC^GGCCGC
1195 GCGGCCGC
PmaCI 1023 CACGTG CAC^GTG
1398 CACGTG
PmlI 1023 CACGTG CAC^GTG
1398 CACGTG
PvuII 589 CAGCTG CAG^CTG
1210 CAGCTG
SapI 138 GCTCTTC GCTCTTC(1/4)
1245 GCTCTTC
StyI 155 CCAAGG C^C[AT][AT]GG
410 CCAAGG
TaqI 818 TCGA T^CGA
872 TCGA
Tsp509I 1094 AATT ^AATT
1447 AATT
TthHB8I 818 TCGA T^CGA
872 TCGA
XhoII 167 AGATCC [GA]^GATC[CT]
1274 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EagI EclXI
Eco52I EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI