Restriction map for
pFN21AE9259
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BciVI
BclI BfrI BlnI BsaBI BsiWI
Bsp1407I BspHI BspLU11I BsrBI BsrGI
Bst1107I BstBI BstZ17I CpoI DraI
DraIII DrdI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MamI MfeI MluI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AccIII 3431 TCCGGA T^CCGGA
AcsI 3013 GAATTT [GA]^AATT[CT]
Aor51HI 2678 AGCGCT AGC^GCT
ApaI 1819 GGGCCC GGGCC^C
ApoI 3013 GAATTT [GA]^AATT[CT]
Asp700I 2503 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 317 GGATCC G^GATCC
BbvCI 3223 GCTGAGG CCTCAGC(-5/-2)
BcgI 3537 CGAAGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 3431 TCCGGA T^CCGGA
BsmI 2514 GAATGC GAATGC(1/-1)
BspDI 2890 ATCGAT AT^CGAT
BspEI 3431 TCCGGA T^CCGGA
BssHII 2518 GCGCGC G^CGCGC
ClaI 2890 ATCGAT AT^CGAT
Eco47III 2678 AGCGCT AGC^GCT
KspI 1093 CCGCGG CCGC^GG
MroI 3431 TCCGGA T^CCGGA
MseI 3017 TTAA T^TAA
NotI 31 GCGGCCGC GC^GGCCGC
PshAI 805 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2892 CGATCG CGAT^CG
SacI 3601 GAGCTC GAGCT^C
SacII 1093 CCGCGG CCGC^GG
SspI 3491 AATATT AAT^ATT
StuI 1562 AGGCCT AGG^CCT
Tru9I 3017 TTAA T^TAA
Tsp509I 3014 AATT ^AATT
XmnI 2503 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 39 enzymes) #
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AflIII 2687 ACGTGT A^C[GA][CT]GT
2923 ACGTGT
AhdI 763 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
856 GACAGCAAGTC
AocI 1133 CCTCAGG CC^T[ACGT]AGG
2839 CCTGAGG
AspEI 763 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
856 GACAGCAAGTC
BalI 671 TGGCCA TGG^CCA
1340 TGGCCA
BbeI 1775 GGCGCC GGCGC^C
2422 GGCGCC
BbrPI 2615 CACGTG CAC^GTG
2922 CACGTG
BbsI 1591 GTCTTC GAAGAC(2/6)
3370 GTCTTC
BglII 666 AGATCT A^GATCT
2096 AGATCT
BlpI 2310 GCTGAGC GC^T[ACGT]AGC
2967 GCTCAGC
Bpu1102I 2310 GCTGAGC GC^T[ACGT]AGC
2967 GCTCAGC
BpuAI 1591 GTCTTC GAAGAC(2/6)
3370 GTCTTC
BsaAI 2615 CACGTG [CT]AC^GT[GA]
2922 CACGTG
BsaI 1399 GAGACC GGTCTC(1/5)
1444 GAGACC
BsaWI 2975 TCCGGT [AT]^CCGG[AT]
3431 TCCGGA
BsrDI 3543 CATTGC GCAATG(2/0)
1376 GCAATG
BssSI 513 CACGAG CACGAG(-5/-1)
1109 CACGAG
BstAPI 1681 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2972 GCATCCGGTGC
Bsu36I 1133 CCTCAGG CC^T[ACGT]AGG
2839 CCTGAGG
CelII 2310 GCTGAGC GC^T[ACGT]AGC
2967 GCTCAGC
EagI 32 CGGCCG C^GGCCG
1670 CGGCCG
Eam1105I 763 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
856 GACAGCAAGTC
EclXI 32 CGGCCG C^GGCCG
1670 CGGCCG
Eco52I 32 CGGCCG C^GGCCG
1670 CGGCCG
Eco81I 1133 CCTCAGG CC^T[ACGT]AGG
2839 CCTGAGG
KasI 1775 GGCGCC G^GCGCC
2422 GGCGCC
MluNI 671 TGGCCA TGG^CCA
1340 TGGCCA
MscI 671 TGGCCA TGG^CCA
1340 TGGCCA
NaeI 1013 GCCGGC GCC^GGC
1672 GCCGGC
NarI 1775 GGCGCC GG^CGCC
2422 GGCGCC
NcoI 1051 CCATGG C^CATGG
2547 CCATGG
NgoMIV 1013 GCCGGC G^CCGGC
1672 GCCGGC
NspI 2067 GCATGC [GA]CATG^[CT]
2405 GCATGC
PmaCI 2615 CACGTG CAC^GTG
2922 CACGTG
PmlI 2615 CACGTG CAC^GTG
2922 CACGTG
PvuII 1278 CAGCTG CAG^CTG
1576 CAGCTG
SfoI 1775 GGCGCC GGC^GCC
2422 GGCGCC
SphI 2067 GCATGC GCATG^C
2405 GCATGC
XcmI 1142 CCAGTGAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1343 CCACTGAGAGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI