Restriction map for
pFN21AB5686
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BlnI BpmI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI KpnI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 1016 TCCGGA T^CCGGA
AcsI 977 AAATTT [GA]^AATT[CT]
AcyI 1318 GGCGTC G[GA]^CG[CT]C
AflII 1420 CTTAAG C^TTAAG
AhdI 191 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 622 CCTCAGG CC^T[ACGT]AGG
ApaI 77 GGGCCC GGGCC^C
ApoI 977 AAATTT [GA]^AATT[CT]
AspEI 191 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 12 GTGCTC G[AT]GC[AT]^C
BanI 739 GGCACC G^G[CT][GA]CC
BbsI 147 GTCTTC GAAGAC(2/6)
BclI 615 TGATCA T^GATCA
BfaI 1376 CTAG C^TAG
BfrI 1420 CTTAAG C^TTAAG
BglI 259 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 563 AGATCT A^GATCT
BpuAI 147 GTCTTC GAAGAC(2/6)
BsaBI 277 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1318 GGCGTC G[GA]^CG[CT]C
BsaWI 1016 TCCGGA [AT]^CCGG[AT]
BseAI 1016 TCCGGA T^CCGGA
BsiEI 82 CGGCCG CG[GA][CT]^CG
BsiHKAI 12 GTGCTC G[AT]GC[AT]^C
BsmBI 1108 GAGACG CGTCTC(1/5)
BspEI 1016 TCCGGA T^CCGGA
BsrDI 754 CATTGC GCAATG(2/0)
BsrI 518 ACTGG ACTGG(1/-1)
BssSI 252 CACGAG CACGAG(-5/-1)
BstXI 759 CCACTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 563 AGATCT [GA]^GATC[CT]
Bsu36I 622 CCTCAGG CC^T[ACGT]AGG
EagI 82 CGGCCG C^GGCCG
Eam1105I 191 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 82 CGGCCG C^GGCCG
Eco52I 82 CGGCCG C^GGCCG
Eco81I 622 CCTCAGG CC^T[ACGT]AGG
FbaI 615 TGATCA T^GATCA
Hin1I 1318 GGCGTC G[GA]^CG[CT]C
MaeI 1376 CTAG C^TAG
MamI 277 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 563 AGATCT [GA]^GATC[CT]
MroI 1016 TCCGGA T^CCGGA
NcoI 1349 CCATGG C^CATGG
PflMI 17 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 43 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 409 CAGCTG CAG^CTG
SapI 1069 GCTCTTC GCTCTTC(1/4)
SbfI 925 CCTGCAGG CCTGCA^GG
SexAI 192 ACCTGGT A^CC[AT]GGT
SmlI 1420 CTTAAG C^T[CT][GA]AG
Sse8387I 925 CCTGCAGG CCTGCA^GG
Tsp509I 978 AATT ^AATT
TspRI 534 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 17 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 89 CCAGGGAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 563 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 1096 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1261 CAGAGCCTG
Aor51HI 174 AGCGCT AGC^GCT
710 AGCGCT
AvaI 170 CCCGAG C^[CT]CG[GA]G
544 CCCGAG
AvaII 44 GGACC G^G[AT]CC
540 GGACC
BalI 266 TGGCCA TGG^CCA
942 TGGCCA
BbrPI 702 CACGTG CAC^GTG
819 CACGTG
BlpI 114 GCTGAGC GC^T[ACGT]AGC
126 GCTGAGC
Bpu1102I 114 GCTGAGC GC^T[ACGT]AGC
126 GCTGAGC
BsaAI 702 CACGTG [CT]AC^GT[GA]
819 CACGTG
BsaI 189 GAGACC GGTCTC(1/5)
1205 GAGACC
BseRI 547 GAGGAG GAGGAG(10/8)
1399 GAGGAG
BsgI 748 GTGCAG GTGCAG(16/14)
997 GTGCAG
BsoBI 170 CCCGAG C^[CT]CG[GA]G
544 CCCGAG
BsrFI 57 GCCGGC [GA]^CCGG[CT]
1253 GCCGGC
CelII 114 GCTGAGC GC^T[ACGT]AGC
126 GCTGAGC
Cfr10I 57 GCCGGC [GA]^CCGG[CT]
1253 GCCGGC
Eco47III 174 AGCGCT AGC^GCT
710 AGCGCT
FauI 48 CCCGC CCCGC(4/6)
136 CCCGC
HaeII 174 AGCGCT [GA]GCGC^[CT]
710 AGCGCT
HincII 642 GTTGAC GT[CT]^[GA]AC
670 GTCAAC
HindII 642 GTTGAC GT[CT]^[GA]AC
670 GTCAAC
KspI 49 CCGCGG CCGC^GG
840 CCGCGG
MaeIII 1120 GTGAC ^GT[ACGT]AC
1321 GTCAC
MluNI 266 TGGCCA TGG^CCA
942 TGGCCA
MscI 266 TGGCCA TGG^CCA
942 TGGCCA
MseI 1187 TTAA T^TAA
1421 TTAA
NaeI 57 GCCGGC GCC^GGC
1253 GCCGGC
NgoMIV 57 GCCGGC G^CCGGC
1253 GCCGGC
PleI 1137 GACTC GAGTC(4/5)
932 GAGTC
PmaCI 702 CACGTG CAC^GTG
819 CACGTG
PmlI 702 CACGTG CAC^GTG
819 CACGTG
SacII 49 CCGCGG CCGC^GG
840 CCGCGG
TfiI 1155 GATTC G^A[AT]TC
1406 GATTC
Tru9I 1187 TTAA T^TAA
1421 TTAA
Tsp45I 1120 GTGAC ^GT[GC]AC
1321 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI TthHB8I