Restriction map for
pFN21AE0462
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MspA1I MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 76 GGCGCC G[GA]^CG[CT]C
AlwNI 43 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 502 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 567 GAGCAC G[AT]GC[AT]^C
BanI 76 GGCGCC G^G[CT][GA]CC
BanII 85 GGGCTC G[GA]GC[CT]^C
BbeI 76 GGCGCC GGCGC^C
BbrPI 232 CACGTG CAC^GTG
BbsI 199 GTCTTC GAAGAC(2/6)
BclI 492 TGATCA T^GATCA
BpuAI 199 GTCTTC GAAGAC(2/6)
BsaAI 232 CACGTG [CT]AC^GT[GA]
BsaHI 76 GGCGCC G[GA]^CG[CT]C
BsgI 340 CTGCAC GTGCAG(16/14)
BsiHKAI 567 GAGCAC G[AT]GC[AT]^C
BsmFI 270 GTCCC GGGAC(10/14)
BspMI 4 GCAGGT ACCTGC(4/8)
BsrDI 597 CATTGC GCAATG(2/0)
BstAPI 42 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 322 GGTGACC G^GT[ACGT]ACC
BstPI 322 GGTGACC G^GT[ACGT]ACC
BstXI 668 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 493 GATC ^GATC
DsaI 149 CCATGG C^C[GA][CT]GG
EcoO65I 322 GGTGACC G^GT[ACGT]ACC
EcoT14I 149 CCATGG C^C[AT][AT]GG
FbaI 492 TGATCA T^GATCA
HgaI 642 GCGTC GACGC(5/10)
Hin1I 76 GGCGCC G[GA]^CG[CT]C
HinfI 507 GATTC G^A[ACGT]TC
KasI 76 GGCGCC G^GCGCC
MboI 493 GATC ^GATC
MslI 228 CATGCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 76 GGCGCC GG^CGCC
NcoI 149 CCATGG C^CATGG
NdeII 493 GATC ^GATC
PmaCI 232 CACGTG CAC^GTG
PmlI 232 CACGTG CAC^GTG
PshAI 147 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 541 GAAGAGC GCTCTTC(1/4)
Sau3AI 493 GATC ^GATC
SfcI 466 CTACAG C^T[GA][CT]AG
SfoI 76 GGCGCC GGC^GCC
SmlI 649 CTCAAG C^T[CT][GA]AG
StyI 149 CCATGG C^C[AT][AT]GG
TfiI 507 GATTC G^A[AT]TC
Tsp509I 349 AATT ^AATT
TspRI 624 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 502 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 60 CGCG CG^CG
475 CGCG
AfaI 170 GTAC GT^AC
279 GTAC
Aor51HI 116 AGCGCT AGC^GCT
545 AGCGCT
BbvI 51 GCTGC GCAGC(8/12)
442 GCTGC
BcnI 272 CCCGG CC^[GC]GG
319 CCCGG
BmyI 85 GGGCTC G[AGT]GC[ACT]^C
567 GAGCAC
BsaI 106 GAGACC GGTCTC(1/5)
145 GAGACC
BsmAI 106 GAGAC GTCTC(1/5)
145 GAGAC
Bsp1286I 85 GGGCTC G[AGT]GC[ACT]^C
567 GAGCAC
BsrI 625 ACTGG ACTGG(1/-1)
483 CCAGT
BstUI 60 CGCG CG^CG
475 CGCG
DdeI 434 CTGAG C^T[ACGT]AG
602 CTCAG
EarI 367 CTCTTC CTCTTC(1/4)
541 GAAGAG
Eco47III 116 AGCGCT AGC^GCT
545 AGCGCT
FauI 479 CCCGC CCCGC(4/6)
27 GCGGG
HapII 273 CCGG C^CGG
320 CCGG
HpaII 273 CCGG C^CGG
320 CCGG
Ksp632I 367 CTCTTC CTCTTC(1/4)
541 GAAGAG
MaeII 233 ACGT A^CGT
301 ACGT
MaeIII 323 GTGAC ^GT[ACGT]AC
644 GTCAC
MspI 273 CCGG C^CGG
320 CCGG
MvnI 60 CGCG CG^CG
475 CGCG
NciI 272 CCCGG CC^[GC]GG
319 CCCGG
NspI 227 GCATGC [GA]CATG^[CT]
417 GCATGC
PpuMI 245 AGGACCC [GA]G^G[AT]CC[CT]
581 GGGTCCT
RsaI 170 GTAC GT^AC
279 GTAC
SphI 227 GCATGC GCATG^C
417 GCATGC
TseI 51 GCTGC G^C[AT]GC
442 GCTGC
Tsp45I 323 GTGAC ^GT[GC]AC
644 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AluI AvaII BsaJI BsiYI
BslI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DraII EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HphI ItaI MboII MnlI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI TaqI TthHB8I