Restriction map for
pFN21AE5127
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaHI BsaI BseAI BsgI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrDI
BsrFI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
DpnII DraI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MboI MboII MfeI MflI
MluI MroI MseI MslI MunI
NaeI NarI NdeI NdeII NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI SacI
SalI SapI Sau3AI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tru9I
TspRI Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 24 GAATTT [GA]^AATT[CT]
AfaI 124 GTAC GT^AC
ApoI 24 GAATTT [GA]^AATT[CT]
BalI 137 TGGCCA TGG^CCA
BanI 205 GGCACC G^G[CT][GA]CC
BanII 163 GAGCCC G[GA]GC[CT]^C
BciVI 284 GGATAC GTATCC(6/5)
BcnI 130 CCGGG CC^[GC]GG
BfaI 265 CTAG C^TAG
BglI 45 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 147 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 147 GCTGAGC GC^T[ACGT]AGC
BsaBI 285 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 281 ACCGGA [AT]^CCGG[AT]
BseRI 186 CTCCTC GAGGAG(10/8)
BsiEI 127 CGGCCG CG[GA][CT]^CG
BsmFI 334 GGGAC GGGAC(10/14)
BspHI 341 TCATGA T^CATGA
BspMI 99 ACCTGC ACCTGC(4/8)
BsrBI 150 GAGCGG CCGCTC(-3/-3)
BssHII 170 GCGCGC G^CGCGC
BstF5I 87 CATCC GGATG(2/0)
CelII 147 GCTGAGC GC^T[ACGT]AGC
CpoI 104 CGGACCG CG^G[AT]CCG
DdeI 148 CTGAG C^T[ACGT]AG
EagI 127 CGGCCG C^GGCCG
EclXI 127 CGGCCG C^GGCCG
Eco52I 127 CGGCCG C^GGCCG
EcoNI 270 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 87 CATCC GGATG(9/13)
HphI 254 TCACC GGTGA(8/7)
KspI 47 CCGCGG CCGC^GG
MaeI 265 CTAG C^TAG
MaeII 116 ACGT A^CGT
MaeIII 118 GTGAC ^GT[ACGT]AC
MamI 285 GATACTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 137 TGGCCA TGG^CCA
MscI 137 TGGCCA TGG^CCA
NciI 130 CCGGG CC^[GC]GG
NcoI 140 CCATGG C^CATGG
NheI 264 GCTAGC G^CTAGC
PvuII 175 CAGCTG CAG^CTG
RcaI 341 TCATGA T^CATGA
RsaI 124 GTAC GT^AC
RsrII 104 CGGACCG CG^G[AT]CCG
SacII 47 CCGCGG CCGC^GG
Tsp45I 118 GTGAC ^GT[GC]AC
Tsp509I 25 AATT ^AATT
XcmI 191 CCATCGGCGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 11 enzymes) #
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BpmI 10 CTGGAG CTGGAG(16/14)
55 CTGGAG
DsaI 47 CCGCGG C^C[GA][CT]GG
140 CCATGG
EaeI 127 CGGCCG [CT]^GGCC[GA]
137 TGGCCA
HaeII 144 GGCGCT [GA]GCGC^[CT]
232 AGCGCC
HapII 130 CCGG C^CGG
282 CCGG
HinfI 65 GACTC G^A[ACGT]TC
310 GAGTC
HpaII 130 CCGG C^CGG
282 CCGG
MspI 130 CCGG C^CGG
282 CCGG
NlaIII 141 CATG CATG^
342 CATG
NlaIV 154 GGGTCC GG[ACGT]^[ACGT]CC
205 GGCACC
PleI 65 GACTC GAGTC(4/5)
310 GAGTC
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I EcoRII EcoT14I FauI Fnu4HI
HaeIII HhaI HinP1I ItaI MnlI
MspA1I MvaI MvnI MwoI Sau96I
ScrFI StyI TseI