Restriction map for
pFN21AB7626
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AhdI Alw44I AlwI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AvrII BalI BamHI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaI BseAI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI HincII HindII
HindIII HpaI Ksp632I MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NlaIII NruI NsiI
NspI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 869 GGTACC G^GTACC
AgeI 837 ACCGGT A^CCGGT
AlwNI 455 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 653 GGGCCC GGGCC^C
Asp718I 869 GGTACC G^GTACC
AspHI 821 GAGCAC G[AT]GC[AT]^C
AviII 395 TGCGCA TGC^GCA
BbrPI 721 CACGTG CAC^GTG
BbsI 238 GAAGAC GAAGAC(2/6)
BpuAI 238 GAAGAC GAAGAC(2/6)
BsaAI 721 CACGTG [CT]AC^GT[GA]
BsaWI 837 ACCGGT [AT]^CCGG[AT]
BsgI 452 GTGCAG GTGCAG(16/14)
BsiHKAI 821 GAGCAC G[AT]GC[AT]^C
Bsp1407I 351 TGTACA T^GTACA
BsrBI 600 GAGCGG CCGCTC(-3/-3)
BsrGI 351 TGTACA T^GTACA
BstAPI 454 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 517 GATC ^GATC
EcoRV 983 GATATC GAT^ATC
FspI 395 TGCGCA TGC^GCA
KpnI 869 GGTACC GGTAC^C
MboI 517 GATC ^GATC
NaeI 229 GCCGGC GCC^GGC
NdeII 517 GATC ^GATC
NgoMIV 229 GCCGGC G^CCGGC
PflMI 907 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 837 ACCGGT A^CCGGT
PmaCI 721 CACGTG CAC^GTG
PmlI 721 CACGTG CAC^GTG
PstI 512 CTGCAG CTGCA^G
PvuII 664 CAGCTG CAG^CTG
Sau3AI 517 GATC ^GATC
SspBI 351 TGTACA T^GTACA
TfiI 660 GAATC G^A[AT]TC
Tsp45I 469 GTGAC ^GT[GC]AC
Van91I 907 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 95 CCAAGAGCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AluI 459 AGCT AG^CT
665 AGCT
BanII 99 GAGCCC G[GA]GC[CT]^C
653 GGGCCC
BglI 67 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
101 GCCCGGTGGGC
BsiEI 65 CGGCCG CG[GA][CT]^CG
203 CGGCCG
BspMI 900 ACCTGC ACCTGC(4/8)
1060 GCAGGT
BstNI 81 CCAGG CC^[AT]GG
123 CCAGG
EaeI 65 CGGCCG [CT]^GGCC[GA]
203 CGGCCG
EagI 65 CGGCCG C^GGCCG
203 CGGCCG
EclXI 65 CGGCCG C^GGCCG
203 CGGCCG
Eco52I 65 CGGCCG C^GGCCG
203 CGGCCG
EcoNI 42 CCTCCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
490 CCTGTGGAAGG
EcoRII 81 CCAGG ^CC[AT]GG
123 CCAGG
HphI 784 GGTGA GGTGA(8/7)
1063 GGTGA
MvaI 81 CCAGG CC^[AT]GG
123 CCAGG
NotI 64 GCGGCCGC GC^GGCCGC
202 GCGGCCGC
PleI 501 GAGTC GAGTC(4/5)
809 GAGTC
PpuMI 83 AGGACCC [GA]G^G[AT]CC[CT]
882 GGGACCT
SfcI 419 CTGTAG C^T[GA][CT]AG
512 CTGCAG
TaqI 324 TCGA T^CGA
846 TCGA
Tsp509I 571 AATT ^AATT
1000 AATT
TthHB8I 324 TCGA T^CGA
846 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AccII AciI AcyI AfaI AvaII
BanI BbeI BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EcoO109I
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
KspI MaeII MaeIII MboII MnlI
MspA1I MspI MvnI MwoI NarI
NciI NlaIV RsaI SacII Sau96I
ScrFI SfoI StyI TseI TspRI