Restriction map for
pFN21AE5004
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# NO CUT ENZYMES ( 108 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
Asp718I AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BspDI BspEI BspHI BsrBI
BsrFI BssHII Bst1107I BstAPI BstUI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII EagI EclXI Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI HapII HindIII HpaII
KasI KpnI KspI MamI MfeI
MluI MroI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 1146 GACGTC GACGT^C
AcyI 1146 GACGTC G[GA]^CG[CT]C
AflIII 723 ACATGT A^C[GA][CT]GT
AhdI 2619 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1451 AGCGCT AGC^GCT
Asp700I 2413 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2619 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2081 GAGCAC G[AT]GC[AT]^C
AspI 1963 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2196 CTCGGG C^[CT]CG[GA]G
BalI 2259 TGGCCA TGG^CCA
BanI 1285 GGTGCC G^G[CT][GA]CC
BcgI 1511 GCACAGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2514 AGATCT A^GATCT
BsaHI 1146 GACGTC G[GA]^CG[CT]C
BsiEI 1168 CGATCG CG[GA][CT]^CG
BsiHKAI 2081 GAGCAC G[AT]GC[AT]^C
BsmI 2684 GCATTC GAATGC(1/-1)
BsoBI 2196 CTCGGG C^[CT]CG[GA]G
BspLU11I 723 ACATGT A^CATGT
BssSI 2579 CACGAG CACGAG(-5/-1)
BstBI 2369 TTCGAA TT^CGAA
CfoI 1452 GCGC GCG^C
DraI 2694 TTTAAA TTT^AAA
DrdI 1731 GACTGTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2259 TGGCCA [CT]^GGCC[GA]
Eam1105I 2619 GACACCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1451 AGCGCT AGC^GCT
EcoRI 742 GAATTC G^AATTC
HaeII 1451 AGCGCT [GA]GCGC^[CT]
HgaI 1747 GCGTC GACGC(5/10)
HhaI 1452 GCGC GCG^C
Hin1I 1146 GACGTC G[GA]^CG[CT]C
HinP1I 1452 GCGC G^CGC
HincII 2041 GTTAAC GT[CT]^[GA]AC
HindII 2041 GTTAAC GT[CT]^[GA]AC
HpaI 2041 GTTAAC GTT^AAC
MluNI 2259 TGGCCA TGG^CCA
MscI 2259 TGGCCA TGG^CCA
NcoI 2343 CCATGG C^CATGG
NdeI 1370 CATATG CA^TATG
NspI 723 ACATGT [GA]CATG^[CT]
NspV 2369 TTCGAA TT^CGAA
PflFI 1963 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1201 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1021 GGGTCCT [GA]G^G[AT]CC[CT]
PvuI 1168 CGATCG CGAT^CG
SfuI 2369 TTCGAA TT^CGAA
Tth111I 1963 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1201 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 45 TCTAGA T^CTAGA
XcmI 1497 CCAGTGGATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2413 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AccI 324 GTCTAC GT^[AC][GT]AC
1969 GTCTAC
AlwNI 1940 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2321 CAGCTCCTG
AseI 2146 ATTAAT AT^TAAT
2305 ATTAAT
AsnI 2146 ATTAAT AT^TAAT
2305 ATTAAT
AvaII 442 GGACC G^G[AT]CC
1022 GGTCC
BamHI 392 GGATCC G^GATCC
1596 GGATCC
BbsI 255 GAAGAC GAAGAC(2/6)
1240 GAAGAC
BpmI 332 CTCCAG CTGGAG(16/14)
1486 CTCCAG
BpuAI 255 GAAGAC GAAGAC(2/6)
1240 GAAGAC
BsaI 276 GAGACC GGTCTC(1/5)
289 GAGACC
BstEII 168 GGTGACC G^GT[ACGT]ACC
958 GGTAACC
BstPI 168 GGTGACC G^GT[ACGT]ACC
958 GGTAACC
BstXI 1814 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2070 CCAGCAGAGTGG
DsaI 1532 CCGTGG C^C[GA][CT]GG
2343 CCATGG
EarI 2248 CTCTTC CTCTTC(1/4)
2417 CTCTTC
EcoO65I 168 GGTGACC G^GT[ACGT]ACC
958 GGTAACC
Ksp632I 2248 CTCTTC CTCTTC(1/4)
2417 CTCTTC
MslI 855 CACTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1641 CACCAAAATG
PshBI 2146 ATTAAT AT^TAAT
2305 ATTAAT
SspI 1837 AATATT AAT^ATT
2656 AATATT
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI BanII BbvI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII