##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TfiI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AciI           57  GCGG                 CCGC(-3/-1)
AclI          315  AACGTT               AA^CGTT
AfaI          139  GTAC                 GT^AC
AlwNI          65  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          211  ATTAAT               AT^TAAT
AsnI          211  ATTAAT               AT^TAAT
Asp700I       109  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         376  GGTCC                G^G[AT]CC
BcnI           83  CCGGG                CC^[GC]GG
BfaI           92  CTAG                 C^TAG
BpmI            7  CTGGAG               CTGGAG(16/14)
BsaJI         132  CCTTGG               C^C[ACGT][ACGT]GG
BsgI           34  GTGCAG               GTGCAG(16/14)
BsiYI         127  CCTTTCCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          127  CCTTTCCTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI          67  GTCCC                GGGAC(10/14)
BsrI           64  CCAGT                ACTGG(1/-1)
BssKI          83  CCGGG                ^CC[ACGT]GG
Cac8I         325  GCCTGC               GC[ACGT]^[ACGT]GC
EcoT14I       132  CCTTGG               C^C[AT][AT]GG
HaeIII         18  GGCC                 GG^CC
HapII          83  CCGG                 C^CGG
HpaII          83  CCGG                 C^CGG
MaeI           92  CTAG                 C^TAG
MaeII         316  ACGT                 A^CGT
MnlI          177  CCTC                 CCTC(7/6)
MspI           83  CCGG                 C^CGG
NciI           83  CCGGG                CC^[GC]GG
PshBI         211  ATTAAT               AT^TAAT
Psp1406I      315  AACGTT               AA^CGTT
RsaI          139  GTAC                 GT^AC
ScrFI          83  CCGGG                CC^[ACGT]GG
StyI          132  CCTTGG               C^C[AT][AT]GG
Tsp509I       356  AATT                 ^AATT
XmnI          109  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BbvI           36  GCAGC                GCAGC(8/12)
              286  GCAGC               
Cfr13I         18  GGCCC                G^G[ACGT]CC
              376  GGTCC               
MseI          212  TTAA                 T^TAA
              218  TTAA                
NlaIV          59  GGCTCC               GG[ACGT]^[ACGT]CC
              375  GGGTCC              
Sau96I         18  GGCCC                G^G[ACGT]CC
              376  GGTCC               
Tru9I         212  TTAA                 T^TAA
              218  TTAA                
TseI           36  GCAGC                G^C[AT]GC
              286  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AluI        Fnu4HI      ItaI        MboII