Restriction map for
pFN21AA1633
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccII AccIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BbeI BbvCI BcgI BlnI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BstUI Bsu36I Cfr10I ClaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PvuI RcaI SacII SalI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 25 GACGTC GACGT^C
AclI 3012 AACGTT AA^CGTT
AcyI 25 GACGTC G[GA]^CG[CT]C
AvaI 5179 CCCGAG C^[CT]CG[GA]G
BanI 2742 GGTGCC G^G[CT][GA]CC
BbrPI 3544 CACGTG CAC^GTG
BciVI 2239 GGATAC GTATCC(6/5)
BcnI 2834 CCCGG CC^[GC]GG
BsaHI 25 GACGTC G[GA]^CG[CT]C
BsaI 4115 GAGACC GGTCTC(1/5)
BsoBI 5179 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2499 TGTACA T^GTACA
BsrGI 2499 TGTACA T^GTACA
Bst1107I 2001 GTATAC GTA^TAC
BstBI 4205 TTCGAA TT^CGAA
BstEII 5235 GGTCACC G^GT[ACGT]ACC
BstPI 5235 GGTCACC G^GT[ACGT]ACC
BstZ17I 2001 GTATAC GTA^TAC
CpoI 3558 CGGTCCG CG^G[AT]CCG
DraI 2932 TTTAAA TTT^AAA
EcoO65I 5235 GGTCACC G^GT[ACGT]ACC
HaeII 5312 AGCGCC [GA]GCGC^[CT]
HapII 2835 CCGG C^CGG
Hin1I 25 GACGTC G[GA]^CG[CT]C
HincII 3812 GTCAAC GT[CT]^[GA]AC
HindII 3812 GTCAAC GT[CT]^[GA]AC
HpaII 2835 CCGG C^CGG
MfeI 5338 CAATTG C^AATTG
MslI 2452 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 2835 CCGG C^CGG
MunI 5338 CAATTG C^AATTG
NciI 2834 CCCGG CC^[GC]GG
NheI 2113 GCTAGC G^CTAGC
NspV 4205 TTCGAA TT^CGAA
PmaCI 3544 CACGTG CAC^GTG
PmlI 3544 CACGTG CAC^GTG
PshAI 2081 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3012 AACGTT AA^CGTT
RsrII 3558 CGGTCCG CG^G[AT]CCG
SbfI 1737 CCTGCAGG CCTGCA^GG
SfuI 4205 TTCGAA TT^CGAA
Sse8387I 1737 CCTGCAGG CCTGCA^GG
SspBI 2499 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccI 2001 GTATAC GT^[AC][GT]AC
2088 GTCTAC
AflII 1957 CTTAAG C^TTAAG
2244 CTTAAG
AflIII 1469 ACGTGT A^C[GA][CT]GT
3543 ACACGT
ApaI 2282 GGGCCC GGGCC^C
3455 GGGCCC
AseI 2161 ATTAAT AT^TAAT
3307 ATTAAT
AsnI 2161 ATTAAT AT^TAAT
3307 ATTAAT
BamHI 872 GGATCC G^GATCC
4405 GGATCC
BfrI 1957 CTTAAG C^TTAAG
2244 CTTAAG
BsaAI 3535 TACGTG [CT]AC^GT[GA]
3544 CACGTG
BsmBI 2857 CGTCTC CGTCTC(1/5)
556 GAGACG
BssSI 616 CACGAG CACGAG(-5/-1)
4112 CACGAG
DrdI 25 GACGTCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3288 GACTGATCAGTC
PpuMI 890 AGGACCT [GA]G^G[AT]CC[CT]
1259 AGGACCT
PshBI 2161 ATTAAT AT^TAAT
3307 ATTAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanII BbsI BbvI BclI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HgaI HhaI HinP1I
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmnI