Restriction map for
pFN21AA0432
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# NO CUT ENZYMES ( 108 enzymes) #
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AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AspEI
AspI AviII AvrII BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
Bst1107I BstAPI BstBI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRV
FseI FspI HaeII HincII HindII
HpaI KasI KspI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 539 GACGTC GACGT^C
Acc65I 1816 GGTACC G^GTACC
AccII 410 CGCG CG^CG
AcyI 539 GACGTC G[GA]^CG[CT]C
AseI 1348 ATTAAT AT^TAAT
AsnI 1348 ATTAAT AT^TAAT
Asp718I 1816 GGTACC G^GTACC
BalI 221 TGGCCA TGG^CCA
BamHI 162 GGATCC G^GATCC
BanI 1816 GGTACC G^G[CT][GA]CC
BsaAI 1390 TACGTG [CT]AC^GT[GA]
BsaBI 385 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 539 GACGTC G[GA]^CG[CT]C
BsgI 1909 GTGCAG GTGCAG(16/14)
BsmFI 2216 GGGAC GGGAC(10/14)
Bsp1407I 1203 TGTACA T^GTACA
BspHI 2192 TCATGA T^CATGA
BsrGI 1203 TGTACA T^GTACA
BssSI 2177 CTCGTG CACGAG(-5/-1)
BstEII 2167 GGTTACC G^GT[ACGT]ACC
BstPI 2167 GGTTACC G^GT[ACGT]ACC
BstUI 410 CGCG CG^CG
BstXI 235 CCAACTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 409 GCGC GCG^C
DraI 2391 TTTAAA TTT^AAA
DsaI 1944 CCATGG C^C[GA][CT]GG
EaeI 221 TGGCCA [CT]^GGCC[GA]
EcoO65I 2167 GGTTACC G^GT[ACGT]ACC
EcoRI 1082 GAATTC G^AATTC
EcoT22I 1609 ATGCAT ATGCA^T
FauI 470 CCCGC CCCGC(4/6)
HgaI 706 GACGC GACGC(5/10)
HhaI 409 GCGC GCG^C
Hin1I 539 GACGTC G[GA]^CG[CT]C
HinP1I 409 GCGC G^CGC
HindIII 1964 AAGCTT A^AGCTT
KpnI 1816 GGTACC GGTAC^C
MamI 385 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 221 TGGCCA TGG^CCA
MscI 221 TGGCCA TGG^CCA
MvnI 410 CGCG CG^CG
NcoI 1944 CCATGG C^CATGG
NsiI 1609 ATGCAT ATGCA^T
PaeR7I 2086 CTCGAG C^TCGAG
PflMI 259 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1348 ATTAAT AT^TAAT
PstI 1087 CTGCAG CTGCA^G
PvuII 578 CAGCTG CAG^CTG
RcaI 2192 TCATGA T^CATGA
SacI 1951 GAGCTC GAGCT^C
SapI 1879 GAAGAGC GCTCTTC(1/4)
ScaI 885 AGTACT AGT^ACT
SexAI 1973 ACCAGGT A^CC[AT]GGT
SspBI 1203 TGTACA T^GTACA
Van91I 259 CCAATCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 464 CCAGAGCCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2086 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AspHI 1831 GAGCAC G[AT]GC[AT]^C
1951 GAGCTC
BbsI 177 GAAGAC GAAGAC(2/6)
2314 GAAGAC
BclI 14 TGATCA T^GATCA
1736 TGATCA
BglII 754 AGATCT A^GATCT
1629 AGATCT
BpuAI 177 GAAGAC GAAGAC(2/6)
2314 GAAGAC
BseRI 1325 CTCCTC GAGGAG(10/8)
617 GAGGAG
BsiHKAI 1831 GAGCAC G[AT]GC[AT]^C
1951 GAGCTC
BsmI 1607 GAATGC GAATGC(1/-1)
1987 GAATGC
DrdI 180 GACAGAATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2388 GACTTTAAAGTC
EcoT14I 663 CCTTGG C^C[AT][AT]GG
1944 CCATGG
FbaI 14 TGATCA T^GATCA
1736 TGATCA
MfeI 422 CAATTG C^AATTG
523 CAATTG
MspA1I 62 CAGCGG C[AC]G^C[GT]G
578 CAGCTG
MunI 422 CAATTG C^AATTG
523 CAATTG
SpeI 844 ACTAGT A^CTAGT
882 ACTAGT
StyI 663 CCTTGG C^C[AT][AT]GG
1944 CCATGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaI AvaII BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BsiYI BslI BsmAI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI