Restriction map for
pFN21AB5054
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FseI
HapII HgaI Hin1I HpaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspA1I MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1056 GTATAC GT^[AC][GT]AC
AccII 80 CGCG CG^CG
AflIII 701 ACGTGT A^C[GA][CT]GT
AlwI 318 GGATC GGATC(4/5)
ApaI 115 GGGCCC GGGCC^C
AseI 874 ATTAAT AT^TAAT
AsnI 874 ATTAAT AT^TAAT
AspHI 644 GAGCAC G[AT]GC[AT]^C
AvaI 41 CCCGAG C^[CT]CG[GA]G
AviII 938 TGCGCA TGC^GCA
BanII 115 GGGCCC G[GA]GC[CT]^C
BclI 1124 TGATCA T^GATCA
BfaI 347 CTAG C^TAG
BglI 31 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 152 CTGGAG CTGGAG(16/14)
BsaBI 570 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 644 GAGCAC G[AT]GC[AT]^C
BsmAI 155 GAGAC GTCTC(1/5)
BsoBI 41 CCCGAG C^[CT]CG[GA]G
BspHI 566 TCATGA T^CATGA
BssSI 775 CTCGTG CACGAG(-5/-1)
Bst1107I 1056 GTATAC GTA^TAC
BstAPI 90 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 80 CGCG CG^CG
BstYI 318 GGATCT [GA]^GATC[CT]
BstZ17I 1056 GTATAC GTA^TAC
FauI 81 GCGGG CCCGC(4/6)
FbaI 1124 TGATCA T^GATCA
FspI 938 TGCGCA TGC^GCA
HincII 64 GTCAAC GT[CT]^[GA]AC
HindII 64 GTCAAC GT[CT]^[GA]AC
HindIII 612 AAGCTT A^AGCTT
MaeI 347 CTAG C^TAG
MamI 570 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 318 GGATCT [GA]^GATC[CT]
MvnI 80 CGCG CG^CG
PflMI 1225 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1012 GACCTAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 874 ATTAAT AT^TAAT
PstI 88 CTGCAG CTGCA^G
RcaI 566 TCATGA T^CATGA
SfiI 30 GGCCGAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1182 CTTGAG C^T[CT][GA]AG
SphI 682 GCATGC GCATG^C
StuI 336 AGGCCT AGG^CCT
Tsp45I 1114 GTCAC ^GT[GC]AC
Van91I 1225 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 318 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 272 GTAC GT^AC
470 GTAC
AlwNI 506 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1226 CAGGTGCTG
Asp700I 334 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1574 GAATGTCTTC
BalI 452 TGGCCA TGG^CCA
530 TGGCCA
BanI 124 GGCACC G^G[CT][GA]CC
1234 GGTGCC
BbsI 12 GAAGAC GAAGAC(2/6)
1578 GTCTTC
BpuAI 12 GAAGAC GAAGAC(2/6)
1578 GTCTTC
BsgI 1521 CTGCAC GTGCAG(16/14)
755 GTGCAG
BsmFI 1032 GGGAC GGGAC(10/14)
1175 GGGAC
BsrI 262 ACTGG ACTGG(1/-1)
1280 CCAGT
DraII 114 GGGGCCC [GA]G^G[ACGT]CC[CT]
386 AGGCCCT
DsaI 639 CCACGG C^C[GA][CT]GG
1238 CCGTGG
EaeI 452 TGGCCA [CT]^GGCC[GA]
530 TGGCCA
EcoO109I 114 GGGGCCC [GA]G^G[ACGT]CC[CT]
386 AGGCCCT
EcoRI 732 GAATTC G^AATTC
999 GAATTC
HaeII 54 GGCGCT [GA]GCGC^[CT]
148 GGCGCT
MaeII 95 ACGT A^CGT
701 ACGT
MluNI 452 TGGCCA TGG^CCA
530 TGGCCA
MscI 452 TGGCCA TGG^CCA
530 TGGCCA
MslI 129 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
579 CATGGAGATG
NspI 682 GCATGC [GA]CATG^[CT]
1035 ACATGC
RsaI 272 GTAC GT^AC
470 GTAC
SfcI 88 CTGCAG C^T[GA][CT]AG
512 CTGTAG
TfiI 850 GATTC G^A[AT]TC
1414 GAATC
XcmI 26 CCAAGGCCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1538 CCAACATTCTCTTGG
XmnI 334 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1574 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrDI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NsiI PleI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp509I TspRI