################################## # NO CUT ENZYMES ( 206 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI Hin1I HincII HindII HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccII 117 CGCG CG^CG AfaI 252 GTAC GT^AC AflIII 254 ACGTGT A^C[GA][CT]GT AlwNI 165 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 243 GGTCC G^G[AT]CC BanII 78 GGGCTC G[GA]GC[CT]^C BmyI 78 GGGCTC G[AGT]GC[ACT]^C BsaAI 253 TACGTG [CT]AC^GT[GA] BsaI 157 GAGACC GGTCTC(1/5) BsaJI 239 CCTGGG C^C[ACGT][ACGT]GG BseRI 28 CTCCTC GAGGAG(10/8) Bsp1286I 78 GGGCTC G[AGT]GC[ACT]^C BsrI 230 CCAGT ACTGG(1/-1) BssSI 31 CTCGTG CACGAG(-5/-1) BstAPI 164 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 117 CGCG CG^CG CfoI 221 GCGC GCG^C Cfr13I 243 GGTCC G^G[ACGT]CC DrdI 15 GACTGTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HhaI 221 GCGC GCG^C HinP1I 221 GCGC G^CGC HindIII 124 AAGCTT A^AGCTT HinfI 235 GAGTC G^A[ACGT]TC MaeII 254 ACGT A^CGT MaeIII 13 GTGAC ^GT[ACGT]AC MspA1I 182 CAGCTG C[AC]G^C[GT]G MvnI 117 CGCG CG^CG NlaIV 242 GGGTCC GG[ACGT]^[ACGT]CC PleI 235 GAGTC GAGTC(4/5) PvuII 182 CAGCTG CAG^CTG RsaI 252 GTAC GT^AC Sau96I 243 GGTCC G^G[ACGT]CC Tsp45I 13 GTGAC ^GT[GC]AC TspRI 231 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### AciI 95 CCGC CCGC(-3/-1) 116 CCGC BfaI 37 CTAG C^TAG 58 CTAG BsmAI 157 GAGAC GTCTC(1/5) 24 GTCTC DdeI 66 CTCAG C^T[ACGT]AG 81 CTCAG MaeI 37 CTAG C^TAG 58 CTAG MwoI 61 GCACTCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 164 GCAGCCTCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AluI BbvI BssKI BstNI Cac8I EcoRII Fnu4HI ItaI MnlI MvaI ScrFI TseI