Restriction map for
pFN21AE1103
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BbvI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI Fnu4HI FseI FspI
HgaI HincII HindII HindIII HpaI
HphI ItaI KpnI KspI MaeIII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI MvnI MwoI NcoI NdeI
NdeII NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI Sau3AI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tru9I TseI Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 307 GGCGCC G[GA]^CG[CT]C
AfaI 177 GTAC GT^AC
AvaI 140 CCCGGG C^[CT]CG[GA]G
AvaII 372 GGTCC G^G[AT]CC
BanI 307 GGCGCC G^G[CT][GA]CC
BanII 390 GAGCTC G[GA]GC[CT]^C
BbeI 307 GGCGCC GGCGC^C
BbsI 193 GAAGAC GAAGAC(2/6)
BbvCI 105 GCTGAGG CCTCAGC(-5/-2)
BfaI 160 CTAG C^TAG
BpmI 321 CTCCAG CTGGAG(16/14)
BpuAI 193 GAAGAC GAAGAC(2/6)
BsaHI 307 GGCGCC G[GA]^CG[CT]C
BsaWI 44 ACCGGA [AT]^CCGG[AT]
BseRI 100 GAGGAG GAGGAG(10/8)
BsmAI 238 GAGAC GTCTC(1/5)
BsmBI 238 GAGACG CGTCTC(1/5)
BsoBI 140 CCCGGG C^[CT]CG[GA]G
BssSI 85 CACGAG CACGAG(-5/-1)
BstF5I 189 GGATG GGATG(2/0)
Cac8I 9 GCCGGC GC[ACGT]^[ACGT]GC
DraII 371 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoNI 340 CCTTCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 371 GGGTCCC [GA]G^G[ACGT]CC[CT]
FokI 189 GGATG GGATG(9/13)
HaeII 307 GGCGCC [GA]GCGC^[CT]
Hin1I 307 GGCGCC G[GA]^CG[CT]C
KasI 307 GGCGCC G^GCGCC
MaeI 160 CTAG C^TAG
MaeII 185 ACGT A^CGT
MspA1I 298 CCGCTG C[AC]G^C[GT]G
NaeI 9 GCCGGC GCC^GGC
NarI 307 GGCGCC GG^CGCC
NgoMIV 9 GCCGGC G^CCGGC
PpuMI 371 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 172 CTGCAG CTGCA^G
RsaI 177 GTAC GT^AC
SacI 390 GAGCTC GAGCT^C
SfcI 172 CTGCAG C^T[GA][CT]AG
SfoI 307 GGCGCC GGC^GCC
SmaI 140 CCCGGG CCC^GGG
SmlI 64 CTCAAG C^T[CT][GA]AG
StuI 362 AGGCCT AGG^CCT
TfiI 48 GAATC G^A[AT]TC
Tsp509I 125 AATT ^AATT
TspRI 216 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 140 CCCGGG C^CCGGG
XmaI 140 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 82 GAGCAC G[AT]GC[AT]^C
390 GAGCTC
BmyI 82 GAGCAC G[AGT]GC[ACT]^C
390 GAGCTC
BsaJI 140 CCCGGG C^C[ACGT][ACGT]GG
368 CCGGGG
BsiHKAI 82 GAGCAC G[AT]GC[AT]^C
390 GAGCTC
Bsp1286I 82 GAGCAC G[AGT]GC[ACT]^C
390 GAGCTC
BsrFI 9 GCCGGC [GA]^CCGG[CT]
270 ACCGGC
BstNI 96 CCAGG CC^[AT]GG
405 CCAGG
CfoI 115 GCGC GCG^C
308 GCGC
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
270 ACCGGC
EarI 198 CTCTTC CTCTTC(1/4)
285 GAAGAG
EcoRII 96 CCAGG ^CC[AT]GG
405 CCAGG
HhaI 115 GCGC GCG^C
308 GCGC
HinP1I 115 GCGC G^CGC
308 GCGC
Ksp632I 198 CTCTTC CTCTTC(1/4)
285 GAAGAG
MvaI 96 CCAGG CC^[AT]GG
405 CCAGG
PleI 196 GACTC GAGTC(4/5)
205 GACTC
SfaNI 211 GATGC GCATC(5/9)
220 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AciI AluI BcnI BsiYI BslI
BsmFI BssKI Cfr13I DdeI HaeIII
HapII HinfI HpaII MboII MnlI
MspI NciI NlaIV Sau96I ScrFI