Restriction map for
pFN21AB8007
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspEI BspHI BsrBI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I Cfr10I CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT22I FseI FspI Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI StuI SwaI
Tth111I Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccII 1633 CGCG CG^CG
AflIII 2665 ACATGT A^C[GA][CT]GT
AseI 1945 ATTAAT AT^TAAT
AsnI 1945 ATTAAT AT^TAAT
AspHI 2587 GAGCTC G[AT]GC[AT]^C
AvaI 2297 CTCGAG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BamHI 1008 GGATCC G^GATCC
BanII 2587 GAGCTC G[GA]GC[CT]^C
BclI 1545 TGATCA T^GATCA
BcnI 1715 CCCGG CC^[GC]GG
BglI 535 GCCAGGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2584 GCTGAGC GC^T[ACGT]AGC
BmyI 2587 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 2584 GCTGAGC GC^T[ACGT]AGC
BsaI 1134 GAGACC GGTCTC(1/5)
BsgI 899 GTGCAG GTGCAG(16/14)
BsiHKAI 2587 GAGCTC G[AT]GC[AT]^C
BsmBI 650 CGTCTC CGTCTC(1/5)
BsmFI 1713 GTCCC GGGAC(10/14)
BsoBI 2297 CTCGAG C^[CT]CG[GA]G
Bsp1286I 2587 GAGCTC G[AGT]GC[ACT]^C
BspDI 2020 ATCGAT AT^CGAT
BspLU11I 2665 ACATGT A^CATGT
BspMI 2557 ACCTGC ACCTGC(4/8)
BsrDI 1583 GCAATG GCAATG(2/0)
BssSI 1520 CTCGTG CACGAG(-5/-1)
BstEII 2648 GGTTACC G^GT[ACGT]ACC
BstPI 2648 GGTTACC G^GT[ACGT]ACC
BstUI 1633 CGCG CG^CG
CelII 2584 GCTGAGC GC^T[ACGT]AGC
CfoI 1782 GCGC GCG^C
ClaI 2020 ATCGAT AT^CGAT
DraI 261 TTTAAA TTT^AAA
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoNI 2569 CCTCTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2648 GGTTACC G^GT[ACGT]ACC
FauI 1900 GCGGG CCCGC(4/6)
FbaI 1545 TGATCA T^GATCA
HaeII 1781 GGCGCT [GA]GCGC^[CT]
HapII 1716 CCGG C^CGG
HgaI 763 GCGTC GACGC(5/10)
HhaI 1782 GCGC GCG^C
HinP1I 1782 GCGC G^CGC
HpaII 1716 CCGG C^CGG
MaeII 1222 ACGT A^CGT
MfeI 1285 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspI 1716 CCGG C^CGG
MunI 1285 CAATTG C^AATTG
MvnI 1633 CGCG CG^CG
NciI 1715 CCCGG CC^[GC]GG
PaeR7I 2297 CTCGAG C^TCGAG
PshBI 1945 ATTAAT AT^TAAT
SacI 2587 GAGCTC GAGCT^C
SphI 2408 GCATGC GCATG^C
XbaI 952 TCTAGA T^CTAGA
XcmI 896 CCAGTGCAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2297 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 1366 GGACC G^G[AT]CC
1612 GGTCC
BsmI 1032 GAATGC GAATGC(1/-1)
2528 GAATGC
BstF5I 737 CATCC GGATG(2/0)
192 GGATG
DsaI 1238 CCATGG C^C[GA][CT]GG
1994 CCATGG
FokI 737 CATCC GGATG(9/13)
192 GGATG
HincII 2176 GTTGAC GT[CT]^[GA]AC
2419 GTTGAC
HindII 2176 GTTGAC GT[CT]^[GA]AC
2419 GTTGAC
HindIII 1648 AAGCTT A^AGCTT
2321 AAGCTT
MslI 502 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1055 CATCAGGGTG
MspA1I 1228 CAGCGG C[AC]G^C[GT]G
1276 CAGCGG
NcoI 1238 CCATGG C^CATGG
1994 CCATGG
NspI 2408 GCATGC [GA]CATG^[CT]
2665 ACATGT
ScaI 823 AGTACT AGT^ACT
2369 AGTACT
SpeI 28 ACTAGT A^CTAGT
820 ACTAGT
SspI 1794 AATATT AAT^ATT
2233 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BfaI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsrI BssKI BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI