##################################
# NO CUT ENZYMES (  218 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DdeI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
ItaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

BbrPI         248  CACGTG               CAC^GTG
BfaI          299  CTAG                 C^TAG
BglII         306  AGATCT               A^GATCT
BsaAI         248  CACGTG               [CT]AC^GT[GA]
BsaJI          42  CCTGGG               C^C[ACGT][ACGT]GG
BseRI         349  CTCCTC               GAGGAG(10/8)
BsmAI         223  GAGAC                GTCTC(1/5)
BsmFI          45  GGGAC                GGGAC(10/14)
BssKI          42  CCTGG                ^CC[ACGT]GG
BstNI          42  CCTGG                CC^[AT]GG
BstYI         306  AGATCT               [GA]^GATC[CT]
DpnII         307  GATC                 ^GATC
EcoRII         42  CCTGG                ^CC[AT]GG
HinfI         172  GAGTC                G^A[ACGT]TC
MaeI          299  CTAG                 C^TAG
MaeII         249  ACGT                 A^CGT
MboI          307  GATC                 ^GATC
MboII          64  GAAGA                GAAGA(8/7)
MflI          306  AGATCT               [GA]^GATC[CT]
MvaI           42  CCTGG                CC^[AT]GG
MwoI           19  GCCATCTTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         307  GATC                 ^GATC
NlaIII        390  CATG                 CATG^
NlaIV         294  GGAACC               GG[ACGT]^[ACGT]CC
PleI          172  GAGTC                GAGTC(4/5)
PmaCI         248  CACGTG               CAC^GTG
PmlI          248  CACGTG               CAC^GTG
Sau3AI        307  GATC                 ^GATC
ScrFI          42  CCTGG                CC^[ACGT]GG
SfaNI         316  GATGC                GCATC(5/9)
SspI           86  AATATT               AAT^ATT
Tsp45I        115  GTCAC                ^GT[GC]AC
XhoII         306  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

BsgI          140  GTGCAG               GTGCAG(16/14)
              251  GTGCAG              
HphI          116  TCACC                GGTGA(8/7)
              149  TCACC               


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BsrI        BstF5I      FokI        MaeIII      MnlI