Restriction map for
pFN21AE1301
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HindIII HinfI HpaI HphI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tsp45I Tsp509I Tth111I TthHB8I
Van91I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AciI 189 CCGC CCGC(-3/-1)
AcyI 316 GGCGTC G[GA]^CG[CT]C
BanI 324 GGCACC G^G[CT][GA]CC
BanII 370 GGGCTC G[GA]GC[CT]^C
BciVI 397 GTATCC GTATCC(6/5)
BfaI 382 CTAG C^TAG
BmyI 370 GGGCTC G[AGT]GC[ACT]^C
BsaHI 316 GGCGTC G[GA]^CG[CT]C
BsaI 83 GAGACC GGTCTC(1/5)
BsaWI 374 TCCGGT [AT]^CCGG[AT]
BsmAI 83 GAGAC GTCTC(1/5)
Bsp1286I 370 GGGCTC G[AGT]GC[ACT]^C
BstF5I 351 CATCC GGATG(2/0)
BstYI 161 GGATCT [GA]^GATC[CT]
Cac8I 119 GCAAGC GC[ACGT]^[ACGT]GC
DdeI 278 CTGAG C^T[ACGT]AG
DsaI 56 CCATGG C^C[GA][CT]GG
EarI 411 GAAGAG CTCTTC(1/4)
EcoT14I 56 CCATGG C^C[AT][AT]GG
FokI 351 CATCC GGATG(9/13)
HaeIII 139 GGCC GG^CC
HapII 375 CCGG C^CGG
Hin1I 316 GGCGTC G[GA]^CG[CT]C
HpaII 375 CCGG C^CGG
Ksp632I 411 GAAGAG CTCTTC(1/4)
MaeI 382 CTAG C^TAG
MflI 161 GGATCT [GA]^GATC[CT]
MseI 22 TTAA T^TAA
MslI 51 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 189 CCGCTG C[AC]G^C[GT]G
MspI 375 CCGG C^CGG
MwoI 363 GCTGGTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 56 CCATGG C^CATGG
NlaIII 57 CATG CATG^
StyI 56 CCATGG C^C[AT][AT]GG
Tru9I 22 TTAA T^TAA
XbaI 381 TCTAGA T^CTAGA
XcmI 47 CCATCATAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 161 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwI 186 GATCC GGATC(4/5)
161 GGATC
AvaII 152 GGACC G^G[AT]CC
245 GGACC
BsrI 115 ACTGG ACTGG(1/-1)
394 CCAGT
Cfr13I 152 GGACC G^G[ACGT]CC
245 GGACC
DpnII 162 GATC ^GATC
186 GATC
MboI 162 GATC ^GATC
186 GATC
MboII 408 GAAGA GAAGA(8/7)
411 GAAGA
NdeII 162 GATC ^GATC
186 GATC
NlaIV 324 GGCACC GG[ACGT]^[ACGT]CC
371 GGCTCC
PstI 70 CTGCAG CTGCA^G
299 CTGCAG
Sau3AI 162 GATC ^GATC
186 GATC
Sau96I 152 GGACC G^G[ACGT]CC
245 GGACC
SfcI 70 CTGCAG C^T[GA][CT]AG
299 CTGCAG
TspRI 114 CACTG CA[GC]TG[ACGT][ACGT]^
182 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstNI EcoRII Fnu4HI HgaI
ItaI MnlI MvaI ScrFI TseI