Restriction map for
pFN21AE3770
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsiWI BsmI BspDI
BspLU11I BsrBI BssHII BssSI BstBI
BstEII BstPI CelII ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NotI NruI NspV
PacI PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SpeI Sse8387I SspI
SwaI Tth111I XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1335 GACGTC GACGT^C
AccI 686 GTATAC GT^[AC][GT]AC
AccIII 921 TCCGGA T^CCGGA
AcsI 2207 AAATTC [GA]^AATT[CT]
AlwNI 2026 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 2207 AAATTC [GA]^AATT[CT]
BamHI 215 GGATCC G^GATCC
BbeI 8 GGCGCC GGCGC^C
BbrPI 2113 CACGTG CAC^GTG
BbvCI 1680 CCTCAGC CCTCAGC(-5/-2)
BcgI 504 GCAGTTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1925 TGATCA T^GATCA
BsaI 523 GAGACC GGTCTC(1/5)
BsaWI 921 TCCGGA [AT]^CCGG[AT]
BseAI 921 TCCGGA T^CCGGA
BsiEI 81 CGGTCG CG[GA][CT]^CG
BsmBI 52 GAGACG CGTCTC(1/5)
Bsp1407I 1776 TGTACA T^GTACA
BspEI 921 TCCGGA T^CCGGA
BspMI 2942 ACCTGC ACCTGC(4/8)
BsrDI 776 CATTGC GCAATG(2/0)
BsrGI 1776 TGTACA T^GTACA
Bst1107I 686 GTATAC GTA^TAC
BstAPI 2025 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 656 CCACTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 686 GTATAC GTA^TAC
DraI 2185 TTTAAA TTT^AAA
EcoRV 607 GATATC GAT^ATC
FbaI 1925 TGATCA T^GATCA
KasI 8 GGCGCC G^GCGCC
MroI 921 TCCGGA T^CCGGA
NaeI 59 GCCGGC GCC^GGC
NarI 8 GGCGCC GG^CGCC
NcoI 840 CCATGG C^CATGG
NgoMIV 59 GCCGGC G^CCGGC
NheI 2758 GCTAGC G^CTAGC
PaeR7I 2583 CTCGAG C^TCGAG
PmaCI 2113 CACGTG CAC^GTG
PmlI 2113 CACGTG CAC^GTG
PvuII 105 CAGCTG CAG^CTG
SfoI 8 GGCGCC GGC^GCC
SnaBI 424 TACGTA TAC^GTA
SphI 2124 GCATGC GCATG^C
SspBI 1776 TGTACA T^GTACA
XhoI 2583 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 31 CGCG CG^CG
56 CGCG
AcyI 8 GGCGCC G[GA]^CG[CT]C
1335 GACGTC
Alw44I 1158 GTGCAC G^TGCAC
2848 GTGCAC
ApaLI 1158 GTGCAC G^TGCAC
2848 GTGCAC
Asp700I 1089 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1797 GAAGCCTTTC
BfaI 647 CTAG C^TAG
2759 CTAG
BsaAI 424 TACGTA [CT]AC^GT[GA]
2113 CACGTG
BsaHI 8 GGCGCC G[GA]^CG[CT]C
1335 GACGTC
BseRI 1355 CTCCTC GAGGAG(10/8)
1765 GAGGAG
BspHI 470 TCATGA T^CATGA
807 TCATGA
BsrFI 59 GCCGGC [GA]^CCGG[CT]
338 ACCGGC
BstUI 31 CGCG CG^CG
56 CGCG
BstYI 215 GGATCC [GA]^GATC[CT]
377 AGATCC
Cfr10I 59 GCCGGC [GA]^CCGG[CT]
338 ACCGGC
DsaI 417 CCGTGG C^C[GA][CT]GG
840 CCATGG
EcoNI 1369 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2279 CCTCCCGCAGG
HgaI 29 GACGC GACGC(5/10)
54 GACGC
Hin1I 8 GGCGCC G[GA]^CG[CT]C
1335 GACGTC
HphI 2634 GGTGA GGTGA(8/7)
2973 TCACC
MaeI 647 CTAG C^TAG
2759 CTAG
MflI 215 GGATCC [GA]^GATC[CT]
377 AGATCC
MvnI 31 CGCG CG^CG
56 CGCG
PflMI 384 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1408 CCAAACATTGG
PshAI 811 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2448 GACATCTGTC
RcaI 470 TCATGA T^CATGA
807 TCATGA
SapI 565 GAAGAGC GCTCTTC(1/4)
342 GCTCTTC
SmlI 2547 CTTGAG C^T[CT][GA]AG
2583 CTCGAG
StuI 1351 AGGCCT AGG^CCT
1733 AGGCCT
Van91I 384 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1408 CCAAACATTGG
XcmI 171 CCAATGCAAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2063 CCAACAGGGGAATGG
XhoII 215 GGATCC [GA]^GATC[CT]
377 AGATCC
XmnI 1089 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1797 GAAGCCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI