Restriction map for
pFN21AF0065
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaII AviII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AfaI 391 GTAC GT^AC
AlwI 486 GATCC GGATC(4/5)
AvaI 76 CCCGGG C^[CT]CG[GA]G
AvrII 547 CCTAGG C^CTAGG
BbsI 196 GAAGAC GAAGAC(2/6)
BbvCI 129 CCTCAGC CCTCAGC(-5/-2)
BfaI 548 CTAG C^TAG
BlnI 547 CCTAGG C^CTAGG
BpmI 28 CTGGAG CTGGAG(16/14)
BpuAI 196 GAAGAC GAAGAC(2/6)
BsoBI 76 CCCGGG C^[CT]CG[GA]G
BstEII 66 GGTGACC G^GT[ACGT]ACC
BstPI 66 GGTGACC G^GT[ACGT]ACC
BstXI 159 CCACTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 485 AGATCC [GA]^GATC[CT]
DpnII 486 GATC ^GATC
DraII 543 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 543 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 66 GGTGACC G^GT[ACGT]ACC
HindIII 406 AAGCTT A^AGCTT
MaeI 548 CTAG C^TAG
MboI 486 GATC ^GATC
MflI 485 AGATCC [GA]^GATC[CT]
NdeII 486 GATC ^GATC
PflMI 49 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 391 GTAC GT^AC
SapI 144 GCTCTTC GCTCTTC(1/4)
Sau3AI 486 GATC ^GATC
SmaI 76 CCCGGG CCC^GGG
TaqI 180 TCGA T^CGA
TthHB8I 180 TCGA T^CGA
Van91I 49 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 45 CCAACCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 485 AGATCC [GA]^GATC[CT]
XmaCI 76 CCCGGG C^CCGGG
XmaI 76 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BcnI 76 CCCGG CC^[GC]GG
77 CCGGG
BseRI 490 CTCCTC GAGGAG(10/8)
22 GAGGAG
BsgI 499 CTGCAC GTGCAG(16/14)
445 GTGCAG
BsrDI 417 CATTGC GCAATG(2/0)
293 GCAATG
BsrFI 63 GCCGGT [GA]^CCGG[CT]
434 ACCGGC
BstF5I 33 GGATG GGATG(2/0)
54 GGATG
BstNI 172 CCTGG CC^[AT]GG
192 CCAGG
Cfr10I 63 GCCGGT [GA]^CCGG[CT]
434 ACCGGC
Cfr13I 169 GGCCC G^G[ACGT]CC
544 GGCCC
EcoRII 172 CCTGG ^CC[AT]GG
192 CCAGG
EcoT14I 237 CCAAGG C^C[AT][AT]GG
547 CCTAGG
FokI 33 GGATG GGATG(9/13)
54 GGATG
HinfI 213 GACTC G^A[ACGT]TC
281 GACTC
MaeIII 67 GTGAC ^GT[ACGT]AC
262 GTGAC
MslI 8 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
160 CACTGCTGTG
MvaI 172 CCTGG CC^[AT]GG
192 CCAGG
NciI 76 CCCGG CC^[GC]GG
77 CCGGG
NlaIII 246 CATG CATG^
442 CATG
PleI 213 GACTC GAGTC(4/5)
281 GACTC
Sau96I 169 GGCCC G^G[ACGT]CC
544 GGCCC
StyI 237 CCAAGG C^C[AT][AT]GG
547 CCTAGG
Tsp45I 67 GTGAC ^GT[GC]AC
262 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BsrI BssKI Cac8I DdeI EarI
Fnu4HI HaeIII HapII HpaII HphI
ItaI Ksp632I MboII MnlI MspI
MwoI NlaIV ScrFI TseI TspRI