Restriction map for
pFN21AE6101
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRII EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MroI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AciI 285 GCGG CCGC(-3/-1)
AfaI 93 GTAC GT^AC
AlwI 135 GATCC GGATC(4/5)
AlwNI 151 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 176 GTGCTC G[AT]GC[AT]^C
BbsI 30 GAAGAC GAAGAC(2/6)
BbvI 52 GCTGC GCAGC(8/12)
BcnI 36 CCCGG CC^[GC]GG
BfaI 140 CTAG C^TAG
BmyI 176 GTGCTC G[AGT]GC[ACT]^C
BpuAI 30 GAAGAC GAAGAC(2/6)
BsiHKAI 176 GTGCTC G[AT]GC[AT]^C
Bsp1286I 176 GTGCTC G[AGT]GC[ACT]^C
BsrI 184 ACTGG ACTGG(1/-1)
BssKI 36 CCCGG ^CC[ACGT]GG
BstYI 134 AGATCC [GA]^GATC[CT]
Cac8I 281 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 40 GCGC GCG^C
Cfr13I 3 GGCCC G^G[ACGT]CC
DpnII 135 GATC ^GATC
EcoT14I 101 CCAAGG C^C[AT][AT]GG
FauI 285 GCGGG CCCGC(4/6)
Fnu4HI 52 GCTGC GC^[ACGT]GC
HaeII 39 GGCGCT [GA]GCGC^[CT]
HapII 37 CCGG C^CGG
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HpaII 37 CCGG C^CGG
HphI 45 GGTGA GGTGA(8/7)
ItaI 52 GCTGC GC^[ACGT]GC
MaeI 140 CTAG C^TAG
MboI 135 GATC ^GATC
MboII 30 GAAGA GAAGA(8/7)
MflI 134 AGATCC [GA]^GATC[CT]
MseI 209 TTAA T^TAA
MslI 240 CACTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 37 CCGG C^CGG
NciI 36 CCCGG CC^[GC]GG
NdeII 135 GATC ^GATC
NlaIV 114 GGTTCC GG[ACGT]^[ACGT]CC
PflMI 83 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 93 GTAC GT^AC
Sau3AI 135 GATC ^GATC
Sau96I 3 GGCCC G^G[ACGT]CC
ScrFI 36 CCCGG CC^[ACGT]GG
SfaNI 290 GCATC GCATC(5/9)
StyI 101 CCAAGG C^C[AT][AT]GG
Tru9I 209 TTAA T^TAA
TseI 52 GCTGC G^C[AT]GC
Tsp45I 58 GTGAC ^GT[GC]AC
Tsp509I 8 AATT ^AATT
TspRI 240 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 83 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 101 CCAAGGTTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 134 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
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BalI 80 TGGCCA TGG^CCA
236 TGGCCA
BsaJI 35 CCCCGG C^C[ACGT][ACGT]GG
101 CCAAGG
BsiYI 36 CCCGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
83 CCACATTTTGG
BslI 36 CCCGGCGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
83 CCACATTTTGG
EaeI 80 TGGCCA [CT]^GGCC[GA]
236 TGGCCA
MaeIII 17 GTAAC ^GT[ACGT]AC
58 GTGAC
MluNI 80 TGGCCA TGG^CCA
236 TGGCCA
MscI 80 TGGCCA TGG^CCA
236 TGGCCA
MwoI 281 GCAAGCGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
290 GCATCATTGGC
TaqI 67 TCGA T^CGA
75 TCGA
TthHB8I 67 TCGA T^CGA
75 TCGA
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# 3 OR MORE CUT ENZYMES ( 4 enzymes) #
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AluI DdeI HaeIII MnlI