Restriction map for
pFN21AB8239
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstF5I BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI FseI HaeII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MroI MseI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfaNI SfcI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 318 CGCG CG^CG
AlwI 240 GATCC GGATC(4/5)
ApaI 45 GGGCCC GGGCC^C
AspHI 401 GTGCTC G[AT]GC[AT]^C
AviII 35 TGCGCA TGC^GCA
BcnI 186 CCGGG CC^[GC]GG
BglI 55 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 223 CTGGAG CTGGAG(16/14)
BsgI 202 CTGCAC GTGCAG(16/14)
BsiEI 53 CGGCCG CG[GA][CT]^CG
BsiHKAI 401 GTGCTC G[AT]GC[AT]^C
BsmBI 96 GAGACG CGTCTC(1/5)
BsmFI 17 GGGAC GGGAC(10/14)
BsrI 14 ACTGG ACTGG(1/-1)
BssSI 404 CTCGTG CACGAG(-5/-1)
BstEII 216 GGTGACC G^GT[ACGT]ACC
BstPI 216 GGTGACC G^GT[ACGT]ACC
BstUI 318 CGCG CG^CG
CfoI 36 GCGC GCG^C
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco52I 53 CGGCCG C^GGCCG
EcoO65I 216 GGTGACC G^GT[ACGT]ACC
FauI 375 GCGGG CCCGC(4/6)
FspI 35 TGCGCA TGC^GCA
HapII 186 CCGG C^CGG
HhaI 36 GCGC GCG^C
HinP1I 36 GCGC G^CGC
HpaII 186 CCGG C^CGG
MboII 106 GAAGA GAAGA(8/7)
MslI 381 CATTGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 186 CCGG C^CGG
MvnI 318 CGCG CG^CG
NciI 186 CCGGG CC^[GC]GG
NlaIII 208 CATG CATG^
PflMI 122 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 278 AGGACCT [GA]G^G[AT]CC[CT]
SexAI 353 ACCAGGT A^CC[AT]GGT
SfiI 54 GGCCGCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 371 TCGA T^CGA
TthHB8I 371 TCGA T^CGA
Van91I 122 CCAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AluI 179 AGCT AG^CT
200 AGCT
AvaII 183 GGACC G^G[AT]CC
279 GGACC
BalI 138 TGGCCA TGG^CCA
284 TGGCCA
BanII 45 GGGCCC G[GA]GC[CT]^C
416 GAGCCC
BsaJI 59 CCCAGG C^C[ACGT][ACGT]GG
221 CCCTGG
BseRI 101 GAGGAG GAGGAG(10/8)
196 GAGGAG
BsrBI 330 GAGCGG CCGCTC(-3/-3)
373 GAGCGG
BstXI 354 CCAGGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
380 CCATTGATGTGG
DdeI 84 CTTAG C^T[ACGT]AG
412 CTGAG
DpnII 240 GATC ^GATC
315 GATC
MaeIII 25 GTGAC ^GT[ACGT]AC
217 GTGAC
MboI 240 GATC ^GATC
315 GATC
MluNI 138 TGGCCA TGG^CCA
284 TGGCCA
MscI 138 TGGCCA TGG^CCA
284 TGGCCA
NdeII 240 GATC ^GATC
315 GATC
NlaIV 45 GGGCCC GG[ACGT]^[ACGT]CC
391 GGAACC
Sau3AI 240 GATC ^GATC
315 GATC
Tsp45I 25 GTGAC ^GT[GC]AC
217 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI BbvI BmyI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI Cac8I
Cfr13I DraII EaeI EcoO109I EcoRII
Fnu4HI HaeIII HphI ItaI MnlI
MvaI MwoI Sau96I ScrFI TseI