##################################
# NO CUT ENZYMES (  205 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BalI        BamHI       
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaJI       BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmFI       BsmI        Bsp1286I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstF5I      
BstNI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        ClaI        CpoI        
DpnII       DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FokI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
ItaI        KasI        KpnI        KspI        MaeI        
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        Sau3AI      SbfI        ScaI        
ScrFI       SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        TaqI        TfiI        TseI        TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI          274  GTAGAC               GT^[AC][GT]AC
AluI          344  AGCT                 AG^CT
AvaI           44  CTCGGG               C^[CT]CG[GA]G
AvaII         447  GGACC                G^G[AT]CC
BanI          487  GGCACC               G^G[CT][GA]CC
BpmI          231  CTGGAG               CTGGAG(16/14)
BsaI          290  GAGACC               GGTCTC(1/5)
BsmBI         160  GAGACG               CGTCTC(1/5)
BsoBI          44  CTCGGG               C^[CT]CG[GA]G
Bsp1407I      101  TGTACA               T^GTACA
BspMI         435  ACCTGC               ACCTGC(4/8)
BsrDI         505  GCAATG               GCAATG(2/0)
BsrFI         490  ACCGGC               [GA]^CCGG[CT]
BsrGI         101  TGTACA               T^GTACA
BstEII        431  GGTTACC              G^GT[ACGT]ACC
BstPI         431  GGTTACC              G^GT[ACGT]ACC
BstXI         356  CCATTTTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        490  ACCGGC               [GA]^CCGG[CT]
Cfr13I        447  GGACC                G^G[ACGT]CC
EarI          340  GAAGAG               CTCTTC(1/4)
EcoNI         495  CCTTGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       431  GGTTACC              G^GT[ACGT]ACC
HapII         491  CCGG                 C^CGG
HpaII         491  CCGG                 C^CGG
HphI          165  GGTGA                GGTGA(8/7)
Ksp632I       340  GAAGAG               CTCTTC(1/4)
MslI          357  CATTTTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          491  CCGG                 C^CGG
NlaIV         487  GGCACC               GG[ACGT]^[ACGT]CC
SapI          340  GAAGAGC              GCTCTTC(1/4)
Sau96I        447  GGACC                G^G[ACGT]CC
SfaNI         256  GATGC                GCATC(5/9)
SmlI           15  CTCAAG               C^T[CT][GA]AG
SspBI         101  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AfaI          102  GTAC                 GT^AC
              171  GTAC                
BsmAI         160  GAGAC                GTCTC(1/5)
              290  GAGAC               
BsrI          230  ACTGG                ACTGG(1/-1)
               29  CCAGT               
DdeI          346  CTGAG                C^T[ACGT]AG
              408  CTCAG               
MaeII         169  ACGT                 A^CGT
              472  ACGT                
MboII         337  GAAGA                GAAGA(8/7)
              340  GAAGA               
RsaI          102  GTAC                 GT^AC
              171  GTAC                
Tsp45I        166  GTGAC                ^GT[GC]AC
              284  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AcsI        ApoI        BsiYI       BslI        HaeIII      
MaeIII      MnlI        MseI        NlaIII      Tru9I       
Tsp509I