Restriction map for
pFN21AA0413
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# NO CUT ENZYMES ( 98 enzymes) #
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Acc65I AccI AccII AccIII AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AvaI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BcnI BlnI BsaWI BseAI BseRI
BsiEI BsiWI BsoBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoT22I FbaI FseI FspI HaeII
HgaI HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmeI PshAI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SphI Sse8387I StuI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AatII 2798 GACGTC GACGT^C
AclI 3574 AACGTT AA^CGTT
AcyI 2798 GACGTC G[GA]^CG[CT]C
Alw44I 950 GTGCAC G^TGCAC
AocI 821 CCTTAGG CC^T[ACGT]AGG
ApaLI 950 GTGCAC G^TGCAC
AspI 3205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1439 TGGCCA TGG^CCA
BanI 1149 GGTGCC G^G[CT][GA]CC
BanII 136 GAGCCC G[GA]GC[CT]^C
BbrPI 3637 CACGTG CAC^GTG
BglI 3341 GCCACAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1705 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1705 GCTAAGC GC^T[ACGT]AGC
BsaBI 2152 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2798 GACGTC G[GA]^CG[CT]C
BsgI 1580 GTGCAG GTGCAG(16/14)
BsmBI 643 CGTCTC CGTCTC(1/5)
Bsp1407I 1656 TGTACA T^GTACA
BspMI 1987 ACCTGC ACCTGC(4/8)
BsrDI 2892 CATTGC GCAATG(2/0)
BsrGI 1656 TGTACA T^GTACA
BstAPI 2530 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 414 GGTCACC G^GT[ACGT]ACC
BstPI 414 GGTCACC G^GT[ACGT]ACC
Bsu36I 821 CCTTAGG CC^T[ACGT]AGG
CelII 1705 GCTAAGC GC^T[ACGT]AGC
CfoI 3357 GCGC GCG^C
DraI 1614 TTTAAA TTT^AAA
DsaI 3492 CCATGG C^C[GA][CT]GG
EaeI 1439 TGGCCA [CT]^GGCC[GA]
Eco81I 821 CCTTAGG CC^T[ACGT]AGG
EcoNI 1131 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 414 GGTCACC G^GT[ACGT]ACC
EcoRI 2019 GAATTC G^AATTC
EcoRV 1364 GATATC GAT^ATC
FauI 1787 CCCGC CCCGC(4/6)
HapII 1678 CCGG C^CGG
HhaI 3357 GCGC GCG^C
Hin1I 2798 GACGTC G[GA]^CG[CT]C
HinP1I 3357 GCGC G^CGC
HincII 2785 GTTGAC GT[CT]^[GA]AC
HindII 2785 GTTGAC GT[CT]^[GA]AC
HpaII 1678 CCGG C^CGG
MamI 2152 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1439 TGGCCA TGG^CCA
MscI 1439 TGGCCA TGG^CCA
MspA1I 2252 CAGCTG C[AC]G^C[GT]G
MspI 1678 CCGG C^CGG
NcoI 3492 CCATGG C^CATGG
NdeI 3659 CATATG CA^TATG
PflFI 3205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 971 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3637 CACGTG CAC^GTG
PmlI 3637 CACGTG CAC^GTG
Psp1406I 3574 AACGTT AA^CGTT
PvuII 2252 CAGCTG CAG^CTG
SnaBI 202 TACGTA TAC^GTA
SspBI 1656 TGTACA T^GTACA
Tth111I 3205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 971 CCAATCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 419 CCATACATGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AflII 2871 CTTAAG C^TTAAG
3625 CTTAAG
AflIII 3636 ACACGT A^C[GA][CT]GT
3638 ACGTGT
AseI 2308 ATTAAT AT^TAAT
2812 ATTAAT
AsnI 2308 ATTAAT AT^TAAT
2812 ATTAAT
Asp700I 1853 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2316 GAAACAGTTC
BfrI 2871 CTTAAG C^TTAAG
3625 CTTAAG
BsaI 677 GGTCTC GGTCTC(1/5)
1666 GGTCTC
EcoT14I 673 CCAAGG C^C[AT][AT]GG
3492 CCATGG
MfeI 2073 CAATTG C^AATTG
3064 CAATTG
MunI 2073 CAATTG C^AATTG
3064 CAATTG
PpuMI 789 GGGTCCT [GA]G^G[AT]CC[CT]
2339 AGGACCC
PshBI 2308 ATTAAT AT^TAAT
2812 ATTAAT
SpeI 2037 ACTAGT A^CTAGT
2449 ACTAGT
StyI 673 CCAAGG C^C[AT][AT]GG
3492 CCATGG
XbaI 1056 TCTAGA T^CTAGA
1917 TCTAGA
XmnI 1853 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2316 GAAACAGTTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI PstI RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII