Restriction map for
pFN21AB5347
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# NO CUT ENZYMES ( 114 enzymes) #
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AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AvrII BalI BamHI BbeI BbrPI
BbvCI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaI BsiEI
BsiWI BsmI Bsp1407I BspDI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI HgaI
HindIII HpaI KasI MfeI MluNI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SspBI
SspI StuI StyI SwaI TfiI
Tsp509I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 520 GACGTC GACGT^C
Acc65I 1241 GGTACC G^GTACC
AccI 16 GTCTAC GT^[AC][GT]AC
AccIII 1295 TCCGGA T^CCGGA
AcyI 520 GACGTC G[GA]^CG[CT]C
AlwNI 1246 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1241 GGTACC G^GTACC
AspI 682 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 605 TGCGCA TGC^GCA
BanI 1241 GGTACC G^G[CT][GA]CC
BcgI 608 GCAGCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 917 TGATCA T^GATCA
BfaI 1124 CTAG C^TAG
BsaAI 19 TACGTG [CT]AC^GT[GA]
BsaBI 864 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 520 GACGTC G[GA]^CG[CT]C
BsaWI 1295 TCCGGA [AT]^CCGG[AT]
BseAI 1295 TCCGGA T^CCGGA
BsgI 262 GTGCAG GTGCAG(16/14)
BsmAI 839 GTCTC GTCTC(1/5)
BsmBI 838 CGTCTC CGTCTC(1/5)
BspEI 1295 TCCGGA T^CCGGA
BspLU11I 77 ACATGT A^CATGT
BspMI 264 GCAGGT ACCTGC(4/8)
BsrDI 596 GCAATG GCAATG(2/0)
BstAPI 93 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 478 CGGTCCG CG^G[AT]CCG
DdeI 407 CTCAG C^T[ACGT]AG
EaeI 442 CGGCCA [CT]^GGCC[GA]
EarI 1233 GAAGAG CTCTTC(1/4)
EcoNI 249 CCTCTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 917 TGATCA T^GATCA
FspI 605 TGCGCA TGC^GCA
Hin1I 520 GACGTC G[GA]^CG[CT]C
KpnI 1241 GGTACC GGTAC^C
Ksp632I 1233 GAAGAG CTCTTC(1/4)
MaeI 1124 CTAG C^TAG
MamI 864 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 221 ACGCGT A^CGCGT
MroI 1295 TCCGGA T^CCGGA
MseI 1031 TTAA T^TAA
PflFI 682 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 417 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 391 CTGCAG CTGCA^G
RsrII 478 CGGTCCG CG^G[AT]CCG
SbfI 390 CCTGCAGG CCTGCA^GG
ScaI 278 AGTACT AGT^ACT
SfcI 391 CTGCAG C^T[GA][CT]AG
SmaI 1080 CCCGGG CCC^GGG
SphI 653 GCATGC GCATG^C
Sse8387I 390 CCTGCAGG CCTGCA^GG
Tru9I 1031 TTAA T^TAA
Tth111I 682 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 417 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 556 CCAGGAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1080 CCCGGG C^CCGGG
XmaI 1080 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 77 ACATGT A^C[GA][CT]GT
221 ACGCGT
ApaI 434 GGGCCC GGGCC^C
1106 GGGCCC
BbsI 1138 GAAGAC GAAGAC(2/6)
640 GTCTTC
BpmI 292 CTGGAG CTGGAG(16/14)
733 CTGGAG
BpuAI 1138 GAAGAC GAAGAC(2/6)
640 GTCTTC
BsmFI 311 GGGAC GGGAC(10/14)
1039 GTCCC
BsrI 753 CCAGT ACTGG(1/-1)
933 CCAGT
BstXI 104 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
956 CCAACAACGTGG
FauI 438 CCCGC CCCGC(4/6)
1104 GCGGG
HaeII 10 AGCGCC [GA]GCGC^[CT]
586 AGCGCC
HincII 535 GTCAAC GT[CT]^[GA]AC
948 GTCAAC
HindII 535 GTCAAC GT[CT]^[GA]AC
948 GTCAAC
KspI 439 CCGCGG CCGC^GG
708 CCGCGG
MaeIII 457 GTCAC ^GT[ACGT]AC
575 GTCAC
MspA1I 439 CCGCGG C[AC]G^C[GT]G
708 CCGCGG
NspI 77 ACATGT [GA]CATG^[CT]
653 GCATGC
PpuMI 686 AGGTCCT [GA]G^G[AT]CC[CT]
1037 GGGTCCC
SacI 346 GAGCTC GAGCT^C
823 GAGCTC
SacII 439 CCGCGG CCGC^GG
708 CCGCGG
Tsp45I 457 GTCAC ^GT[GC]AC
575 GTCAC
TspRI 754 CAGTG CA[GC]TG[ACGT][ACGT]^
1164 CAGTG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsoBI Bsp1286I BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MflI MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI TthHB8I
XhoII